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We narrowed to 295 results for: SAM activators

Showing: 161 - 180 of 295 results

  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...  LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose cannot be taken...increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced...convoluted due to researchers sharing and renaming samples throughout history. It was likely isolated in 1918...taken up by cell mcrA, mcrBC Inactivation of pathway that cleaves methylated cytosine DNA Allows for ... foreign (methylated) DNA mrr, Δ(mcrC-mrr) Inactivation of pathway that cleaves methylated adenine or...source leuB β-isopropyl malate dehydrogenase inactivated Requires exogenous leucine source for growth ...proAB lacIqZΔM15 Tn10(TetR Amy CmR)]  Note: Inactivating mutations in specific genes are signified with...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...chemogenetics (Designer Receptors Exclusively Activated By Designer Drugs, DREADDs) and optogenetics as...use of DREADDs (Designer Receptors Exclusively Activated By Designer Drugs) to determine the role of serotonin...chemogenetic receptors delivered by AAV within the same cell types. Not only can we compare these methods...finger nucleases, and many others (12). The most attractive potential of AAVs is their ability to be used...McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary recombinant adeno-associated...PMC190186. 9. Grieger, Joshua C., and Richard J. Samulski. "Packaging capacity of adeno-associated virus...

  4. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...enzyme that has been mutated to block its catalytic activity and instead become trapped in a covalent bond ...brightness, stability, or other properties is an active area of research. If size is a concern for your...sequentially adding different ligands to the same sample to label sub-populations of your protein of interest...probably find lots of different plasmids containing the same protein of interest tagged with different fusion...affinity purification tags, another version with that same red fluorescent protein somebody made because they...ligands (Gautier et al., 2008). Although based on the same original enzyme, SNAP-tag and CLIP-tag show good...specificity for their own ligands with minimal cross-reactivity. Other strategies include using engineered ligases...

  5. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...A) Betacyanin is produced from tyrosine by the activity of 3 enzymes and a spontaneous step. (B) RUBY ...GUS staining or are not suited to studying large samples. RUBY Red But recently, something new has been ...consisting of these three enzymes expressed under the same promoter with self-cleaving 2A site linkages and...transformation next to an untransformed seed from the same plant beginning to germinate. (Top right, second...

  6. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ...invited to speak to other Addgenies. I could take an active role in the company and not just feel like an additional... I pondered on the ways that I wouldn't have the same experience as actually being in the office. However...

  9. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...proteins in tissues, cells, and biofluids. Recently, Sam Myers, a Scientist in the group, developed a CRISPR-based...and RNA sequencing of complex heterogeneous RNA samples. Furthermore, such high processivity enzymes and...specific genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase...Using these lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed from...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...). Reprogramming site-specific retrotransposon activity to new DNA sites. Nature. doi: https://doi.org....eB capsid, to express either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato...variants as iGluSnFR4f (fast deactivation) and iGluSnFR4s (slow deactivation). Both have significantly improved...indicators with increased sensitivity and tailored deactivation rates Addgene's own empty lentiviral backbones...indicators with increased sensitivity and tailored deactivation rates By Mike LacyGlutamate is one of the most...indicators with increased sensitivity and tailored deactivation rates. bioRxiv, 2025.03.20.643984. https://doi.org...proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG), and are (typically) tethered to the...

  11. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...downstream analysis focuses on the DNA your protein is actively interacting with, reducing background information...you’re working with a tissue sample, you’ll need to homogenize your sample before moving on to the next...Homogenization for a tissue sample can also need significant optimization for the sample type and size.) It can...process is very similar for both, though tissue samples do require an extra homogenization step. For this...to focus on the ChIP process and not the upsteam sample collection process or the downstream analysis process...itself begins at...  Crosslinking Once you have your sample, the first step is to ensure your protein:DNA complexes...likely to remain attached to the DNA throughout the sample processing and therefore will require crosslinking...

  12. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...accelerating the translational impact of research activities. By streamlining the development and accessibility... able to beat this virus, we will need to do the same with science,” said Guillaume Bourque, a Professor...

  14. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ... influence receptor conformation and signaling activity​​ (Sridharan et al., 2014). It's also crucial ...addition of fluorophores affects the functional activity of the ligands. For instance, studies have shown...cytoplasmic background signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes....org/10.1038/s41592-021-01338-6 Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C.,...

  15. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...knockout or edit. This can help reduce off-target activity. Deleting a large region of the genome by removing...transcriptional repressor), and dCas9-VP64 (transcriptional activator)-containing plasmids. Each destination vector ...multiplexed gRNAs? By expressing multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets...situation: you only need to express two gRNAs at the same time. One system you could use is pX333 from the...options that are available from Addgene follow these same assembly principles, but they’re optimized for different...

  16. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...gene’s open reading frame, in fact, they can be activated a considerable distance away from a gene which...which GFP was monitoring was being expressed in the same pattern. These systems suggest the type of phenotype...

  19. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...from AAV2 because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs can be...cis-acting elements, which means that they are in the same DNA molecule as the gene that they package. In a.... M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated Virus Serotype-Specific...

  20. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...non-small cell lung cancer cell line containing an activating mutation in epidermal growth factor receptor ...replicates would contain the same labels - i.e. they would always come from the same parent cells that represent...replicate groups, and treated these groups with the same drug regimen, they would find one of two outcomes...population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance...
Showing: 161 - 180 of 295 results