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Showing: 161 - 180 of 471 results

  1. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...website resources that best match a user’s query, and return the matching resources in an order that makes sense...expose as filters and designed the search engine to return only the filters that are relevant to a specific...

  2. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...few commonly available different fragments that retain antigen binding (Figure 2):  F(ab')2 - These fragments...fragments are produced by proteolytic digestion that retains an antibody’s disulfide bonds within the hinge ...

  3. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such as human growth...microenvironments! Take myoepithelial cells for instance. They retard tumor formation by inhibiting cell growth, invasion...

  5. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ... and RNA is retained in the top, aqueous layer, while DNA and other proteins are retained in the interphase...

  6. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and have...to permanently induce expression? Check out our Retro expression vector and Lenti expression vector. ...

  7. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...fiction story that doesn’t require any detailed retention of information or formulation of new knowledge...mental bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise...

  8. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...demonstrate SEND as a modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA...

  9. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...plan for a career outside academia (that would be pretty much all scientists), a LinkedIn profile is absolutely...rules of good resume writing. Include examples of concrete accomplishments in both the technical and “plus...

  11. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...gender analysis and 2) provides case studies with concrete examples of how gender analysis leads to discovery...design. But eyes have been opened—and we cannot return to a world that ignores gender. Many thanks to...

  13. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ... body and thereby correct the mutations using retroviral vectors. However, many diseases require techniques...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...

  14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...particularly effective as it can result in the retention of introns or the removal of exons. Disrupting...microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. doi:10.1080...

  15. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...Resources References Demanèche, S., Bertolla, F., Buret, F., Nalin, R., Sailland, A., Auriol, P., Vogel,...

  16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...

  18. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...than pre-designed sets, so identical searches can return multiple answers. You might want to take advantage...fragments, or defined split regions. SplitSet will then return the highest-fidelity overhang set it can find for...

  19. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...quantification of various intermediate steps, a sizing electrophoretic gel, and qPCR quantification of the final libraries...assembly. Most of the time, these plasmids are returned as one or two partial assemblies. If we obtain...

  20. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...access it later. Pro tip Checking the number of retweets, likes, and comments on a tweet about a paper ...papers are connected via citations, in order to return results that are more relevant to your search terms...
Showing: 161 - 180 of 471 results