RaPID Detection of RNA-protein Interactions
Type
Blog Post
Published
Aug. 16, 2018, 12:40 p.m.
...preserves RNA structure and naturally occurring complexes present in the cell, it can also introduce non-physiological...interest. See figure 1 below for details on the key components of RaPID.
Ramanathan et al. also tweaked BirA... BirA* homolog found in B. subtilis. BASU was compared head-to-head with the E. coli and BioID2 BirA*...streptavidin signal (~250-fold enrichment) when compared to a scrambled RNA motif control). BioID2 had ...with BASU still generated a >30-fold enrichment compared to E. coli’s BirA*. Faster labeling kinetics also...HHCS-associated sequences had lower IREB2 binding enrichment compared to wild-type sequences and that loss of binding...Russell, P., & Guttman, M. (2014). Methods for comprehensive experimental identification of RNA-protein interactions...