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Showing: 161 - 180 of 499 results
  1. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your Next Manuscript 6 Tips...
  2. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...is an intense amount of competition between microorganisms for available resources. This competition drives...
  3. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden...
  4. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...around the world, such as BosLab here in Boston. Organizations like this one are always looking for scientists... own independent research!). There are also organizations like Science from Scientists, which try to bring...
  5. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...
  6. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...distinct subcellular environments, including the organelles that compose the secretory pathway, endocytic...formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible to these non-native modifications... Lee Snapp. "Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and...
  7. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...non-human primates [7, 8], zebrafish [9], and c. elegans [10]. If used properly, optogenetics is sure to...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...neural activity in freely moving Caenorhabditis elegans." Nature methods 8.2 (2011): 147-152. PubMed PMID...
  8. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...the observation of proteins in living cells and organisms. Both components of the chimera are encoded by...). However, these assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini...hindrance by the fluorescent protein. Thus, scientist began using relatively long linkers between the protein...
  9. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... were identified using systematic evolution of ligands by exponential enrichment (SELEX). After 4-6 rounds...single molecules and for live-cell imaging in C. elegans. Other groups have used Mango to study RNA-protein...PubMed Central PMCID: PMC3852148. Warner, Katherine Deigan, et al. "Structural basis for activity of highly...
  10. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ... to living cells thus APEX is not suitable for organoid or in vivo studies (Che and Khavari, 2017). Find...many fusion proteins this would lead to protein/organelle aggregation. The Ting lab hypothesized that the...Efficient proximity labeling in living cells and organisms with TurboID." Nature biotechnology 36.9 (2018...
  11. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...has also been implemented in a variety of other organisms, such as sphingomonads and bacillus.  ADH1 The...shown that the Q system is also functional in C. elegans. Subsequent work by the Luo and Potter labs has...repressible binary expression system in Caenorhabditis elegans.” Nat Methods 9(4) (2012): 391-5. PubMed PMID: ...
  12. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...signaling molecules, transcription factors, and ligands. The presenter then showed which genes they had...that were essential for the activity of different ligands. Such knowledge could be very useful for drug discovery... to block the cellular response to a particular ligand (such as when a component of a microbe or a cytokine...
  13. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ... blood stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms...Cancer cells also produce programmed cell death ligand (PD-L1) that inhibits the cytotoxic activity of...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells...
  14. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ... MC, Metzger A, Titus KR, Gilgenast TG, Gong W, Beagan JA, Raj A, Phillips-Cremins JE (2019) LADL: light-activated...Biology. Springer US, pp 261–281 Manna D, Maji B, Gangopadhyay SA, Cox KJ, Zhou Q, Law BK, Mazitschek R, Choudhary...for remote-controlled genome editing of internal organs and tumors. Sci Adv 6:eabb1777 . https://doi.org...
  15. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ...from Science in the News, a graduate student organization that works to promote science communication....non-scientists. These programs are all run and organized by graduate student researchers. That’s right,...
  16. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    Published
    May 24, 2016, 2:30 p.m.
    ...Plant Breeding, Genetics, and Biotechnology at Michigan State University with the backing of a prestigious...:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10:29 : Kingdom's transition...
  17. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ... data on the date, material, requesting PI & organization, and country. This information can be of personal...data only, information on requesting PI(s) & organization(s) is not shown on the dashboard. Key features...
  18. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making Your Own Competent...101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick...
  19. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...healthcare worker with symptoms. The World Health Organization says the number of new cases could rise to 10,000... Kendall Morgan...
Showing: 161 - 180 of 499 results