Antibodies 101: Stripping and Reprobing Western Blots
Type
Blog Post
Published
Aug. 1, 2024, 1:15 p.m.
...incubation at 37 °C.
Wash the membrane for 5–10 minutes 2–3x in fresh wash buffer such as PBS or TBST.
Stringent...Purpose
Tris HCl
62.5 mM
Buffer
SDS
2% (w/v)
Detergent
Β-mercaptoethanol (βME)
100...minutes at 50 °C.
Wash the membrane for 5–10 minutes 2–6x in fresh wash buffer such as PBS or TBST. Note ...better than a nitrocellulose membrane.
If you have already run your blot using nitrocellulose, you may decide...protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what...instructions for the membrane you are using.
Dry nitrocellulose membranes are brittle, so proceed with caution...