Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
Type
Blog Post
Published
Oct. 11, 2018, 12:30 p.m.
...Golden Gate assembly involves the simultaneous activities of a Type IIS restriction enzyme such as BsaI...base pair substrates, it demonstrates significant activity on some mismatch-containing pairings. During Golden...confirmed the insertion of the Lambda insert at the same high frequencies as the antibiotic selection markers...