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  3. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...     Additional Resources Learn how you can use FRET to study protein interactions Learn about other ...CRISPR plasmids for isolating a genomic locus Browse FRET plasmids for studying protein interactions  ...

  6. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ...

  8. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...In most cases, the resulting chimeric antibody retains the binding specificity to the original target ...In addition, chimeric antibodies will inevitably retain some sequence from their parental species which...

  9. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...be the first author. When I raised this, he was pretty angry and started arguing with me. First I calmed...jumping backwards in time to explain something, rethink your structure. Develop your protagonist In most...

  10. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ... XP and melanoma more broadly. “This [award] is pretty great for us,” Wang said. “We were planning on ...mutations in the gene may prevent neurons from returning to basal conditions normally after periods of ...

  11. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s axon. While...even larger area, thus necessitating even higher retrograde AAV titers for successful transduction. In another...

  12. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    .... Over the last twenty years, we’ve developed a pretty smooth system for this process. We often get positive... sent the MTA to sign. Once they've signed and returned the MTA, Addgene can move forward with shipping...

  13. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...sequencing is successful, your NGS provider should return results in the form of many, many short DNA sequences...specific libraries, but any analysis script should return the same output: a spreadsheet of read counts. ...

  14. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...Peak emission (nm) Size (kDa) Substrate Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455...cell membrane and deliver the substrate; however, secreted luciferase or the use of alternative substrate...

  16. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...envelope protein for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids...as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates...

  17. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application...attenuated, nef-deleted HIV-1 strains in vivo. Retrovirology. 2007 Sep 23;4:66. doi: 10.1186/1742-4690-4-...

  18. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...plot. In this post, I’ll walk you through how to interpret the antibody readout on a flow plot.  Note that...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...

  19. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... and sequence displays are now much easier to interpret and analyze at a glance. For a quick look at just...“Analyze Sequence” button. The maps hosted here retain all the features shown in the static maps discussed...

  20. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...quickly." Final design (right). Immediate and immense regret of the graphic designer not shown.   However... and knowledge changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid...
Showing: 141 - 160 of 471 results