Addgene

We narrowed to 254 results for: INO

Showing: 141 - 160 of 254 results

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Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

Type

Blog Post

Published

June 27, 2018, 1:16 p.m.

...biotin–phenol and H2O2, APEX2 labels electron-rich amino acid side chains (e.g. Tyrosine) with biotin. These...
Summer SciComm Series: Master of SciComm

Type

Blog Post

Published

July 21, 2022, 1:15 p.m.

...once again write for fun eventually saw an English minor tacked onto my BSc. With this decision I had unwittingly...
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

Type

Blog Post

Published

Nov. 12, 2019, 2 p.m.

...active up to 100°C. The IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22...
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

Type

Blog Post

Published

Oct. 10, 2019, 12:41 p.m.

... translation in U2OS cells. HA is small, only 9 amino acids, thus it is translated quickly by the ribosome...
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Type

Blog Post

Published

June 9, 2016, 2:30 p.m.

...affect editing efficiency. Smaller size At only 887 amino acids in length, NgAgo is shorter than SpCas9 (1368...
Plasmids 101: A Brief History of Plasmids and an Improved eBook!

Type

Blog Post

Published

Oct. 29, 2015, 2:30 p.m.

...into the E. coli chromosome in some cases. This terminology held until the 1960s when scientists began to...
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

Type

Blog Post

Published

April 7, 2022, 1:15 p.m.

...pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol anchor. SGZ contains a PDGFR transmembrane...
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

Type

Blog Post

Published

June 1, 2021, 1:15 p.m.

...professionals, joined the platform; while they are a minority, their help has been essential to push certain...
New and Upcoming Viral Vectors - September 2019

Type

Blog Post

Published

Sept. 12, 2019, 1 p.m.

...(105322-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary inhibitory neurotransmitter...
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

Published

Sept. 3, 2019, 1:15 p.m.

...lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal...
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

Type

Blog Post

Published

July 27, 2017, 1:11 p.m.

... enhanced “pdDronpa” domains could be placed in amino acid loops on either side of an enzyme’s active ...
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

Type

Blog Post

Published

Sept. 19, 2017, 1:20 p.m.

...just 1 minute of exposure. MG-2I-dL5** experienced minor bleaching after longer exposure times. Additionally...
Summer SciComm Series: A PhD in Science Communication

Type

Blog Post

Published

Aug. 23, 2022, 1:15 p.m.

...scientific methodology makes it easier to spot even minor weakness or limitations in a piece of research (...
Plasmids 101: Gram Negative and Positive Bacteria

Type

Blog Post

Published

Aug. 2, 2022, 1:15 p.m.

...University, who is majoring in marine biology and minoring in studio arts photography. References and resources...
What Good Citizenship Can Do for Reproducibility in Science

Type

Blog Post

Published

Nov. 7, 2019, 2:18 p.m.

... can never completely eliminate the risk that a minor variation in experimental conditions will derail...
Components of CRISPR/Cas9

Type

Blog Post

Published

Feb. 2, 2016, 5 p.m.

...aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium ...
Antibodies 101: Isotypes

Type

Blog Post

Published

Oct. 19, 2021, 1:15 p.m.

...have different versions or subclasses that have minor differences in their heavy chain constant domains...
Sharing is Caring: But How to Distribute Open Hardware?

Type

Blog Post

Published

Feb. 28, 2023, 2:15 p.m.

... available tools such as the Raspberry Pi, the Arduino, or 3D printers are key to enabling this work. ...
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Type

Blog Post

Published

June 25, 2019, 12:54 p.m.

...lab. Chemicals like calcium phosphate and diethylaminoehtyl (DEAE)-dextra neutralize or even impart an...
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

Type

Blog Post

Published

Sept. 26, 2024, 1:15 p.m.

...The mixed PCR and incomplete PCR methods yield a minority of usable product with two 5′ overhangs, so these...

Showing: 141 - 160 of 254 results

Results per page:

Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

Summer SciComm Series: Master of SciComm

Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Plasmids 101: A Brief History of Plasmids and an Improved eBook!

New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

New and Upcoming Viral Vectors - September 2019

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

Summer SciComm Series: A PhD in Science Communication

Plasmids 101: Gram Negative and Positive Bacteria

What Good Citizenship Can Do for Reproducibility in Science

Components of CRISPR/Cas9

Antibodies 101: Isotypes

Sharing is Caring: But How to Distribute Open Hardware?

Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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