... effects on actin filament length (lower). d) Intensity and filament length traces for a different event...benzylguanine (BG) (Keppler et al., 2003). This engineered hAGT later became known as SNAP-tag. There is...cross-reactivity. Other strategies include using engineered ligases to attach a ligand to a peptide tag on... ligands are available from suppliers like New England Biolabs and Promega (though if you are comfortable..., M., Beaufils, F., & Johnsson, K. (2008). An Engineered Protein Tag for Multiprotein Labeling in Living...

...GeoCas9 started with an interesting question: why engineer a new Cas9 when you can just find one that naturally...naturally occurs? This is different than the engineered approach used to make eSpCas9 and SpCas9-HF1. By... PAM Sequence NGG CRAA (R = A or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal Activity...prior research had shown that a cocaine esterase engineered to have increased thermostability also had a ...Read about CRISPR Methods for Bacterial Genome Engineering   Interested in using CRISPR/Cas9 RNPs? Check...

...up with all these questions, Dr. Le Cong and Dr. Feng Zhang developed a Google Forum to allow scientists... the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems has expanded out of ...wildtype or double nickase for my CRISPR genome engineering experiments? A1: When assessing which nickase...nickase type to use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized... Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure 4 of the Cong, ...

...competitive skill sets in a myriad of professions is to engage in extracurricular activities (EA). EAs can: Introduce...Introduce new perspectives in science and engineering Looking at your research from a broader viewpoint...expands your professional network. No brainer! Engaging audiences from across the hall, fields, and other...traditional academic setting and to shape and strengthen interests and skills. Although the word “extracurricular...potential EAs and which professional skill sets are strengthened. This short list highlights the ubiquity of ...

.... This is like taking an engine out of the car – you can still use the engine as a power source, but now...experiments for FD-CF reactions can be fun and engaging for students. In our BioBits kit for example, ... a 4th year PhD student at MIT in Biological Engineering and is interested in developing synthetic biology... biology Use the pathways over time plasmids to engage students in functional genomics research Learn ...

...by New England Biolabs, Inc. Many thanks to our guest blogger Lydia Morrison from New England Biolabs...contributed by guest blogger Lydia Morrison from New England Biolabs. What is DNA assembly? In the context of...the NEBuilder® HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows...becomes hard to accurately execute. However, these challenges can be circumvented by generating individual ...

... NIR light.  NIR wavelengths have better tissue penetration than shorter wavelengths, which makes it possible...will light up if excited with light of specific wavelength and also produce ROS - targeted cell ablation...iodine-modifications also shift MG-2I’s excitation wavelength to 666 nm, which is within the near-infrared ...non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could be used with these...

... that both the peak excitation wavelengths and peak emission wavelength of the two proteins should be ...with shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like...the best protein for a given application is a challenge. To help keep you abreast of the latest fluorescent...

...components of EvolvR and its potential applications. Challenges of existing mutagenesis methods Several continuous...drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...recombineering and Multiplex Automated Genome Engineering (MAGE) will always be limited by the size of ...investigate non-coding segments of the genome, or engineer microbes to perform tasks. EvolvR could also be...about other CRISPR methods for bacterial genome engineering   Learn more about lambda red recombineering ...

...insoluble granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones...editing for psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins...community-centric science, our team has continuously engaged with a variety of stakeholders from industrial ...harmonious progress.     We’re always delighted to engage with potential collaborators and interested audiences...synthetic biology experiment Read our Genome Engineering Guide ...

...algorithms, such as SIFT or PolyPhen to approach this challenge, using attributes of cancer variants such as evolutionary...to making cancer precision medicine a reality. Challenges for cancer variant characterization For many ...validation experiments. But, there have been two huge challenges preventing us from testing this hypothesis: ...Accelerator Plasmid Collections To overcome this first challenge, we’ve developed the Broad Institute Target Accelerator...cancer variants To overcome the second major challenge, we developed assays to test large numbers of ...

...management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...all”. Each team member has unique motivations, strengths, and learning styles. For example, during my time...have led to frustration, micromanagement, and disengagement. By tailoring my leadership approach to each...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...team meeting to highlight their complementary strengths. By encouraging alignment on goals and clarifying...

...Sample6 still leverages the core engineering and scientific concepts to engineer bacteriophages and use those... As a biomedical engineer and scientist, I like to envision that my research will have a direct impact...himself from the idea of starting a company around engineered bacteriophages. He decided to quit his "safety...his co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...

...recombineering-based MAGE (Multiplex Automated Genome Engineering) technology. pMA7CR_2.0 expresses lambda Red ...References 1. Maddalo, Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with ..., et al. “Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids.... Sakuma, Tetsushi, et al. “Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector...plasmids from this paper at Addgene. 5. Tsai, Shengdar Q., et al. “Dimeric CRISPR RNA-guided FokI nucleases...tools for efficient germline and somatic genome engineering in Drosophila.” Proceedings of the National Academy...

...are ~27kD engineered fusion proteins similar to a Fab fragment but created through engineering instead ...reduced-in-size options often have reduced binding strength, half-lives, and/or specificity as well. They’... repeat proteins, or DARPins, are genetically engineered small affinity proteins, around 14-15 kDs in ...produced in E. coli, and are both soluble and easily engineered.  Monobodies are ~10kD synthetic binding proteins...

... more about how you approach new challenges.     Know your strengths! Research experience presents ample...matter of applying that framework to the myriad challenges we encounter. As you spend time in a lab learning...yourself if you missed any important details. Or challenge yourself to think of more creative solutions. ...employer that you are well equipped to work through challenges from day one in your new position. Of course,...

...elegans Neuronal Gene Expression Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene...characterize the transcriptome. The first data release for CeNGEN is planned for August 15th, 2019. For more information...information about the aims and approach of CeNGEN, check out their publication.   3’UTRome The 3’UTRs of...popular! References Hammarlund, Marc, et al. "The CeNGEN Project: The Complete Gene Expression Map of an...

...Here's what you'll find in this post: Epigenetic engineering with single-pot assembly Tracking cell-cell interactions...New items from the viral service Epigenetic engineering with single-pot assembly By Eric Perkins Though...epigenetic researchers custom design their own engineering plasmid tools in about a week. Taking advantage...H3K27me3), a linker between these modules (linker length can and should be varied as it can have important...in AAV1!  New ready-to-use AAV prep from Hongkui Zeng's lab are available for dual expression of cytoplasmic-tdTomato...

... sensor (GEX), where one wavelength changes with Ca while another wavelength is insensitive. Additional...requiring only a simple measurement. However, single wavelength measurements are more susceptible to artifacts...calcium reporter derived from troponin C. It is engineered with a reduced number of calcium binding sites...Cre-dependent expression. These sensors have also been re-engineered to measure changes in calcium specific to the...

...Most things in biology are not so simple, and engineering complex cellular behaviors requires making complex...output, etc., which can support a larger cellular engineering goal, several of which are described below. Multigene...sometimes many times), and isolated afterwards. To engineer these production pathways, MoClo has been used... an array of distinct goals within metabolic engineering, synthetic product production, and gene editing...  References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular...