CRISPR 101: Multiplex Expression of gRNAs
Type
Blog Post
Published
Sept. 10, 2020, 11:45 a.m.
...restriction sites flanking the promoter-gRNA construct, but with different sequences adjacent to the sites. When...genome by removing the sequence between two target sites.
Modifying multiple genes at once. Using multiplexed...promoter-gRNA constructs in order to target multiple sites! In this post, we'll cover Cas9 multiplexing options...oligonucleotides. These plasmids contain Type IIS restriction sites that flank the promoter-gRNA constructs. When these...unique overhangs (here, O1-4) adjacent to the cut sites “link” fragments together and drive ordered assembly...transcribed together and are flanked by specific sites that allow them to be cleaved and released. These...smaller than constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors...