The PAM Requirement and Expanding CRISPR Beyond SpCas9
Type
Blog Post
Published
Aug. 20, 2020, 2:30 p.m.
...Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered CRISPR-Cas9...-associated virus (AAV).
At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is even...first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1), a nuclease that generates...Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science 368:290–296 . https... genome engineering...