Plasmids 101: Using Transposons in the Lab
Type
Blog Post
Published
July 6, 2021, 1:28 p.m.
...in viral vectors, but easily fit in a TE. Transposons are also less likely than viral vectors to induce...encodes a Type I-F CRISPR-Cas system. This system has four major components: 1) a CRISPR RNA (crRNA), 2) ...Non-autonomous transposons, however, need another TE to express a transposase or reverse transcriptase so they ...transposon’s LTRs, similar to when packaging viral vectors.
Let’s take a closer look at three different ... in human cells at rates similar to retroviral vectors. The hyperactive version of the SB transposase,...efficiency in human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold...transfer
Transposons are an alternative to viral vector delivery of transgenes, like for iPSC reprogramming...