... The benefits of nanobodies over conventional antibodies can’t be overlooked. Nanobodies are smaller in...easier to clone, express, and purify compared to antibodies. Nanobodies are easily expressed in the cytosol...

...additional data regarding the specificity of this antibody?” You will also help troubleshoot products, give... in a Western Blot protocol and then blame the antibody when no signals are obtained. Or someone may heat...

...Fluorogen activating proteins (FAPs) – These modified antibody fragments bind and enhance small molecule fluorogens...Nevertheless, these fluorophores were designed as antibody and oligonucleotide labels for fixed-cell imaging...

...component of vaccines and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended...

...elucidate these interactions scientists have long used antibody based affinity purification and mass spectrometry...proteins, which can be a challenge for classic antibody based affinity purification/mass spectrometry ...

...sequences are highly homologous, preventing design of antibodies specific to each fluorophore. Third, Brainbow...mKate2.) They then successfully generated custom antibodies to each of these proteins and confirmed that ...

...Multimerization in the SunTag system occurs via antibody-peptide labeling; specifically, cognate peptide...recognized and thus fluorescently labeled by scFv antibodies fused to sfGFP. This system amplifies the intensity...scaffold containing epitopes recognized by scFV antibodies fused to VP64 transcriptional activation domains...more - all without the need to develop custom antibodies. Unfortunately, while there are many types of...proteins, ordered structures, and accessibility to an antibody. After identifying possible protein domains containing...containing these properties, they generated monoclonal antibodies against them, fused them to GFP, and compared...

...characterization, these proteins could function as surrogate antibodies for identifying and characterizing sugars and...temperature. If you’re lucky enough to have an antibody or other protein on hand that will specifically...

...GCN4. GCN4 recruits GFP fused to the cognate scFV antibody, which is expressed from a separate plasmid. This...

...others to develop an anti-tumour therapy using antibodies targeting dendritic cells. These genetic tools...

...Activin A. A commercially developed therapeutic antibody that binds Activin A and interferes with inappropriate...

... or purify it – and you do not have a specific antibody against the protein available – you can always...

... rabbit (EMD, AB3596) and mouse (NEB, E8032S) antibodies against MBP are commercially available. Factor...

...that can recruit GFP fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the...

...of glycoproteins for human therapies, such as antibodies, is possible in K. phaffii since it produces ...

...There were, of course, many companies selling antibodies, microscopes, and dissection tools, but there...

...South African company wanted to test if a type of antibodies from an Andean mammal called alpaca could neutralize...

...15 kDa), single monomeric variable heavy chain antibody domain.  How do these components interact? When...

...receptor. The receptor could be something like an antibody, enzyme, DNA, etc. The receptors job is to generate...

...including 1) cellular responses in addition to antibody response 2) very high immunogenicity 3) and long-lasting...