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Showing: 121 - 140 of 270 results

  1. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...solution. PEG takes several hours on a heated stir plate to dissolve and requires monitoring so that the ...plasmid, not that I would know about this… A no template control (NTC) should always be included. While...

  2. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ... vector and your insert, DpnI digest away the templates, and transform the PCR products into E. coli. ...will require a PCR reaction or two to generate templates for the cloning process. Time spent actually doing...

  3. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... on Friday. It’s best to split cells before the plates are more than 80-85% confluent because cells like...Benjamin Franklin)! It’s good practice to label your plates and/or flasks with your name, date, the cell line...

  4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...experiment worked just by looking at the cells on the plate. For example, if your plasmid contains a gene encoding... Chromoprotein expression from bacteria on agar plate. Image: Liljeruhm et al., 2018. For each chromoprotein...

  5. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...universities!) This guide tells you how to use online platforms, such as Zoom, to determine if a program is the... yours does and then use that time! Different platforms have different quirks, so remember to practice...

  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...signaling in primary cells. The authors used this platform to assay dozens of GPCRs across many cell types...context-dependent GPCR activity with a universal biosensor platform. Cell, 187(6), 1527–1546.e25. doi: https://doi.org...

  7. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...Cutting both strands ensures that there isn’t a template strand left behind that the cell can use to repair...combination of chewing up one stand and cutting the template strand causes large deletions of up to hundreds...

  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...may lead to loss of the plasmid, and using older plates could increase plasmid loss or mutation. Let it...smaller cultures, especially those in deep-well plates, require a faster shaking speed for proper aeration...

  9. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...publication. Addgene makes this easy by providing a template citation for you to use in your Materials and ... Use the Addgene catalog number to easily find template citations for Addgene plasmids you have used in...

  11. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...is a factor in AAV titration since the starting template is the purified viral vector solution (which contains...be mitigated by using purified viral DNA as the template instead of the intact AAV particle. Finally, ...

  12. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum discussions...to see the questions first makes Reddit a great platform for those who aren’t so great at coming up with...

  13. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...amplification. Transformed E. coli are allowed to grow on plates or in liquid culture to amplify the library, followed...Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale...

  14. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...Addgene launched our AAV Data Hub as a community platform for the sharing of that exact data. Here, requestors...develop new systems, new partnerships, and new platforms, from our early engagement with UPenn to our launch...

  15. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...modifiers available. This system utilizes an RNA template containing the desired edit adjoined to the sgRNA...affect gene expression? dCas9 can be used as a platform to deliver various cargo to a specific DNA locus...

  16. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...concentrations of lactate. The microfluidic screening platform developed here is also a promising approach for...other targets. Figure 6: A) Schematic of BeadScan platform for screening candidate biosensor variants for...

  17. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...Validating knock-in mutations You’ve introduced a donor template along with your gRNA and Cas9 combo and are hoping...Jonkers, Bas van Steensel. Easy quantification of template-directed CRISPR-Cas9 editing. (2018) Nucleic Acids...

  19. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...materials and information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized ...Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein interactions...

  20. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...associated with an elevated mutation burden. The CRISPR platform must be optimized to reduce off-targets and patient...Hopefully you can understand that depending on your platform, off-target effects should impact your experimental...
Showing: 121 - 140 of 270 results