Skip to main content
Addgene
Showing: 121 - 140 of 294 results

  1. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions for storage...Resources on the Addgene Blog Learn All about Adenovirus Associated Virus New to Virus? Check out Our ...

  2. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...postdoc positions, including AstraZeneca, Pfizer, Novartis, Biogen, Amgen, New England Biolabs, Regeneron...pre-approved project. Other companies, such as Novartis, have specific web sites for postdoc listings,...

  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    .... https://doi.org/10.1074/jbc.RA120.01289     Novel protein cage provides simple enzyme protection in...Gabrielle Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting proteins in yeast...

  5. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...) system functions by harnessing the power of a novel degrader of the 12 kDa protein FKBP12F36V. By fusing...can be degraded by this selective degrader. The novel degrader, dTAG13, is a small molecule which induces...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...When contamination is suspected, we proceed to de novo assembly of the unmapped reads, employing our bioinformatics...Hanley L, Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation Sequencing and Analysis Platform...

  7. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability...surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can form a complex with...

  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...incubation can be skipped by pre-binding to 1° Monovalent Monoclonal Only anti- rabbit and -mouse versions...Lee, C., Pleiner, T., Schliep, J.E., Stark, H., Trakhanov, S., & Urlaub, H. (2015). Nanobodies: site-specific...

  9. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...ligands. New fluorophores As a corollary to these innovative labeling strategies, several groups—including...infusion of photons for your imaging experiment. Innovative labeling strategies and improved fluorophores...

  11. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...program should “enable the ability to study life’s innovations from anywhere in the tree of life.” Accepted ... biomass). The lab is developing a system using novel vesicles to efficiently transform these cells which...

  12. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... by our partners at the Institute for Protein Innovation (IPI), part of a new collection of epitope tag...Aryana Khosravani Deborah Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption...

  13. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified...Determination of Lentiviral Infectious Titer by a Novel Droplet Digital PCR Method. Human Gene Therapy Methods...

  14. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...choice. ThermoFischers GeneArt Gene Synthesis NovoPro Labs Codon Optimization Tool (ExoOptimizer) Genewiz...Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out Addgene'...

  15. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...neurons are activated when the mouse is placed in a novel context. As a proof-of-principle test, the authors...show that HR blocked neuronal activation in the novel context as determined by reduced c-fos expression...

  16. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...capable of inducing the brightest fluorescence (Filonov et al., 2014). Broccoli is twice as bright as Spinach2...9.10 (2014): 2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection ...

  17. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...19 testing methods.   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR...for rapid, ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies...

  18. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...are not easily reversible. Late last year, the Akhmanova Lab published a new optogenetic tool for localized...Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova, A. (2022). Opto-katanin, an optogenetic tool ...

  19. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...e.g. mScarlet) or infrared fluorescent proteins (Chernov et al., 2017) may improve detection. Since longer...24360272. PubMed Central PMCID: PMC3918502.  14. Chernov, Konstantin G., et al. "Near-infrared fluorescent...

  20. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...papers or are brainstorming new technological innovations. Let’s take a look at three historical techniques...past. In the sequencing world, one up and coming innovation is nanopore-based DNA sequencing which reads ...
Showing: 121 - 140 of 294 results