...collection is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available...our entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors... testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged in various serotypes. This...recently released these controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad...broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs ubiquitous expression...interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter...mCherry in the absence of Cre, and expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium ...

...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have...

...via phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly...

...fluorescent protein topics page Get the basics on GFP Find microbiology blog posts Resources on Addgene.org...variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal entry site and...a variant has wild-type levels of abundance, the eGFP will be stable and fluoresce. But if the variant...unstable, it will be degraded, leading to reduced eGFP fluorescence. Cells expressing the library are then...fluorescence-activated cell sorting into four bins based on their eGFP:mCherry ratios. Genomic DNA from each bin are then extracted...

...library comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order...

...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, all...

...be another method to assess transfection success. GFP is often used as a reporter and we will be covering...

...selection cassette. They could also switch from FLAG to GFP or a different epitope. Addgene: Do you have any ...

...cells in vivo, the non-immunogenic violet-excited GFP variant Vex. To understand the impact of this gene...

...line, Hep3B. From left to right: Mitotracker Red, GFP for the biosensor, Hoechst, and overlay).   ...(DKFZ) who has developed new redox sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses..., Pedre Pérez’s biosensor fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore...the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and change its fluorescence properties...

...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...

... for a researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA ...

...Expression with Cre-lox Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids...

... enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One caveat...

...Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre....

...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple...

...select both over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio...

..., Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral...

...check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular...

...which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial ...