...transformed without a heat-shock step. Because of the large number of transformations being performed at Addgene...

... possible to quickly and efficiently assemble a large number of building blocks with just two or three...

...counter-selection. Anecdotally, there is also a large amount of user-to-user variability, and in some ...modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise point mutations...

...companies now sell fast digest enzymes that can digest large amounts of DNA in as little as 10 minutes, but check...Additionally, if your final product is going to be very large (>10kb) you might want to use electro-competent ..., the DNA migrates toward the anode (+ charge). Larger fragments of linearized DNA migrate slower than...purification you can do is a miniprep, but if you need larger quantities of DNA, you’ll need to do a midiprep...

...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...

... responsible for cancer from genomics data, and large-scale patient cohort data from the MD Anderson Cancer...

...1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets already...CRISPR/Cas9. You check the mutation and it causes a large deletion that removes the entire exon that codes...

...migration resistance and improve separation. For large proteins, reduce the percentage of acrylamide. To... and typically takes less than an hour. However large proteins typically do not transfer as well using...

...mutations behind. Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated...recombinase. However, the use of negative selection largely overcomes this obstacle. Furthermore, we have found...a cell line with the edit of interest. With the larger piggyBac™ donor vector, it is possible to make ...

... no good treatment. The dystrophin gene is very large (79 exons), but much of the sequence appears to ...lifespan. This work should be done in mouse and large animal models with mutations in human-relevant exons...

...correct phages will test. These includes labs with a large number of different phages or with phages targeting...

...physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting that scientists...the more friends they have in common. Only the largest connected part of the follower network is shown...

...single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold fully occupied with GFP has...

...system allowed us to handle and distribute such a large deposit. Once the project is complete, Ginkgo will...

... or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small (Rep52 and Rep40) Rep...

...collaborations and conferences for their students. Another large goal of the organisation is to encourage iGEM alumni...

...follow their passion. “There are many ways to have a large positive impact in society. We need much, much more...

... nm will pass through animal and human tissues largely unimpeded. The challenge then was to develop fluorescent... Ultrasound wavelengths, on the other hand, are larger than the size of a cell, so they scatter much less...

.... John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification in...

...interest.   Advantages Disadvantages Can carry large transgenes (>8 kb) Potential for generation of replication-competent...have been developed, but these vectors are quite large and you might get lower viral titer when using them...