...and PNS Resources on Addgene.org Find the Spaghetti Monster probes at Addgene Browse all AAV vectors...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...University. Find the Spaghetti Monster probes at Addgene Uses of smFPs 1. Multi-channel connectomic tracers...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...PubMed PMID: 18057027. Additional Resources on the Addgene Blog Rabies and neuronal tracing Retrograde AAV...

...Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene’s CRISPR Guide Page Find... editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms...obligate thermophiles without the need to grow the organisms at lower than optimal temperatures. GeoCas9’s ...try, as always, the plasmids are available from Addgene. Happy CRISPRing! References Harrington, Lucas...bioRxiv (2017): 138867. Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome... Find CRISPR plasmids at Addgene ...

...below and an Addgenie will help you find what you need.   More Plasmid Resources from Addgene: Plasmids... is which vector will best suit your needs? At Addgene, we have a vast collection of empty backbones that...your expression system or environment. The host organism will determine the type of vector that you will...your plasmid has been incorporated into the host organism, usually achieved with the proper selection marker... of the specific mutations. For these purposes Addgene has a large collection of yeast expression vectors...Plasmids 101 blog series Addgene's Molecular Cloning Guide & Other Plasmid Protocols List of common epitope ...

...skills”. Leadership is prized at all levels of an organization and is also one of the most common criteria ...work alone much of the time, depending on the organization they lead. Effective leaders rarely give orders...series of blog posts in one pdf - download the Addgene Management for Scientists Ebook.  Fire, Snowball...

...Dimers and Multimers" post. It's also been re-organized and updated with a fresh new look.  We think this...plasmids, they don’t go to Wikipedia; they go to Addgene.”-Ryan Mehl, Professor, Oregon State University...

...grow liver stem cells and liver-like mini-organs or organoids, neither of these can substitute for a liver...career blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our career videos ...talk preparation: Define your research vision. Organize your vision into a chalk talk format. Practice...questions from open ended screens and data collection. Organize your research vision into the chalk talk format...outdoor photographer.  Additional resources on the Addgene blog Find tips for presenting a science talk Check...

...Genetic Engineering Resources on Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory...and is relatively easy to grow, it is an ideal organism for teachers looking to expand their science curriculum...professional education associations, and local organizations such as the Center for Science and Industry ...-12 education.     Additional Resources on the Addgene Blog Learn how BioBuilder can bring Synthetic ...

... viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral...and space. Zebrafish have become a popular model organism because their larval stage lends itself well to...toolbox and the versatility of the zebrafish model organism, the Isacoff lab was able to link these cells ...PMCID: PMC2770190.  Additional resources on the Addgene blog Find all our optogenetics blog posts Read...

... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...integration, transposon, and many more! Check out Addgene’s catalog to find trapping tools for your next experiment...Plasmids 101 e-book Additional resources on the Addgene blog Plasmids 101: Using Transposons in the Lab....1105/tpc.12.7.1007  Additional resources on Addgene.org Vector database Intro to the Lab Bench Plasmids...

...collaboration.  In that post, we talked about Addgene’s data processing needs, our criteria for selecting... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our...Recently, Seqera published a case study on how Addgene has used Nextflow to improve and redesign our pipeline...

...on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our Hot Plasmids Articles to See Compiled...

... Additional resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...

...abundant biological dark matter and so the lab organized a sampling trip to Yellowstone National Park (...then, many researchers have searched for novel organisms and enzymes from these extreme environments. Gearing...particularly useful. Additional resources on the Addgene blog Learn about other Cas proteins Read all of...GeoCas9, another thermostable Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for...

...For example, the many resources available on the Addgene website didn't come into being through the actions...develop to reach their full potential in the organization. This will result in more engaged, successful... Managerial Skill" Additional Resources on the Addgene Blog Read the Rest of the Management for Scientists...Series Learn How Volunteering at a Professional Organization Can Help You Develop Leadership Skils Set Concrete... Individual Development Plan Resources on the Addgene Website Are You a Molecular Biologist? Find Plasmids...

...a positive stir and was recognized by meeting organizers in their town hall.  Iterative improvement - ...Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance than others... with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons to...take some leaps. Binning Singletons is its own organization, working to make scientific meetings more productive...Binning Singletons, Inc. Additional resources on the Addgene blog Browse our blog posts on scientific conferences...

...Read Other Inside Addgene Posts Resources on the Addgene Website Meet the Addgene Management Team Join...Additional Resources on the Addgene Blog Get Management Advice from Addgene Executive Director Joanne ... are needed. All-Company Meetings: As your organization grows, so will the length of your group meetings...meetings. Make sure to keep them manageable. At Addgene, teams can add as many points as they like to the...the meeting, take notes on the agenda points. At Addgene, we like to do this live on the screen using a ...review or project plans, before the meeting. At Addgene, we like to send around a Google doc where people...provided some tips above for what works well at Addgene, but meetings are never one-size-fits all. Feel...

...CRISPR plasmids for that particular organism available at Addgene. Percentages represent the fraction ... are members of the Addgene Advisory Board. Additional Resources on the Addgene Blog Get the Most from...available to the academic research community. At Addgene, we’ve helped distribute many of these technologies...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...Biologically Inspired Engineering and member of the Addgene Advisory board, on the difficulty of producing ...John Doench (Broad Institute and member of the Addgene Advisory Board) says: "When you're setting up to...Getting these quality-controlled reagents from Addgene can greatly expedite a researcher's ability to ...

...tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098...small, and their transparency allows for whole organism imaging. They created an endogenous ApoB-NanoLuc...cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International Journal of...systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications, 2019. PubMed...worms and have made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ...

...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...gestures are memorable! Attend events for one organization or go to the same conferences/meetings over ... up will be easy. Additional resources on the Addgene blog Browse other blog posts on networking Attending...

...sharing your plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study chromatin...associated PI pages on the Addgene website. In-exhaustive List of Addgene Depositors Who Study Chromatin...pii: 1722491115. Addgene blog posts related to chromatin This is not the first Addgene blog post about ... 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium. As it happens, there are...phenomenon, there’s a reasonable good chance that Addgene can help. Though it would be difficult to consolidate...Keystone Chromosome Architecture and Chromosome Organization. These aren’t the only investigators in the ...the chromatin field who have deposited with Addgene, but you’ll find many great tools by clicking on the...