...the polyA sequence is inverted only in the Cre-expressing tissues. This inversion creates a template that...Yoo BB, et al. Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

...protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator where the...available for distribution. If your experiment involves delivering a calcium biosensor using AAV, you ...

...process. The majority of current cloning techniques involve the assembly of a circular plasmid in vitro, before...Laboratory of Molecular Biology. He is currently investigating the molecular biology of synaptic transmission...and plasticity.     Javier García-Nafría is an investigator scientist at the MRC Laboratory of Molecular...

...want the works: organizing reagent storage and inventory, managing orders, tracking students and research...access your protocols at the bench, keep your inventory at your finger tips, and make shopping lists as...protcols, sign-up for shared equipment, and track inventory and orders. Quartzy caters to life science labs...

...of collecting fibroblasts, which require a more invasive skin biopsy procedure. This becomes a major benefit...grade hiPSCs, reprogramming methods that do not involve modification of the original genome by integrating...cell. The method also reduces biosafety concerns involved in production and usage of Sendai virus. Reprogramming...

... This will give you a feeling of what might be involved in such a role. In addition, it can be advantageous...extent there is the need (or opportunity) to get involved in product testing in the lab, to travel to customers...outreach, and more. Technical support roles also involve interacting with other departments such as research...

...Scrum is that it provides transparency to everyone involved about the status of each project. This creates...example, we recently reduced the number of steps involved during checkout. Demo details for this would be...writing code for common tasks, it would be like re-inventing the wheel over and over again for every single...

...cell lines can become cross contaminated and are invariably much older than those available from biorepositories... NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent identification...Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen Cell Culture Basics Guide Abcam Cell Culture ...

...scientist. I recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (... students. You can see why I was excited to be invited to attend. My job is to ensure that, in general...

... of conflicts is important for the individuals involved, and also for the labs, organizations, or businesses... when you know that a person or team should be involved in making a decision and you see no record of ... means the other party will feel punished or invalidated. Both compromise and arbitration might remedy...

... genes mutated in human cancers have a homolog involved in yeast cell division (Pray, 2008). Scientific...common protein found in lewy bodies), have been invaluable in understanding the basic molecular mechanisms...other model organisms. Worms have been used to investigate an array of biological systems and processes ...

...2013). Since then, Spinach2 has been used to investigate intracellular viral DNA dynamics in neuronal ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed of... blog Learn more about aptamer fluorophores Investigate other fluorescent biosensors Find other uses ...

...means to manage their work, combining data, team, inventory and project management functionalities with experiment...protocols. To facilitate this, some ELNs allow you to invite people and assign them roles and permission that... all data is organized within an ELN already. Investing some time in deploying a software solution that...

...Hendel et al. 2015). Low detection rates mean the investigator can be relatively certain that their samples...off-target mutations are essential data points for investigators looking to definitively link genotype and phenotype...genome-wide unbiased methods. However, most investigators use single cell clones for in vitro CRISPR experiments...

...and optogenetics meet, allowing scientists to investigate the fundamental basis of both brain function ... flip-excision) switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation))...recognition sites, its presence induces first opsin inversion, and then lox sites excision, therefore locking...

...nucleases, recombinases) which irreversibly excise or invert a specific chunk of DNA. However, a limitation ...genome-engineering. One particularly attractive application involves using dCas9-activator fusion proteins to induce...a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven challenging ...identifying previously unknown factors and pathways involved in pre-mRNA splicing in a compound screen. ...visualize the fusion in vitro or in vivo. The protocol involves isolating total RNA, resolving by PAGE and then...

...Stability of fluorescence: For applications that involve ranges of pH, and significant lengths of time, ...incorporation of fluorophores, as seen in studies involving dopamine D2/D3 receptors (Allikalt et al., 2020...processes like mitochondrial dynamics. This process, involving mitochondrial homeostasis maintained through mechanisms...

...yet powerful way to tag cellular proteins and investigate protein localization, interaction, and expression... of the most important applications for PA-FPs involve enhancing optical live imaging of proteins, organelles...and cells. Similar to FPs, PA-FPs allow for non-invasive labelling, but in contrast, PA-FPs offer a reduced...

... Singapore. Chapter 4: Life as a new primary investigator. Coming up with new project ideas, applying ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab...

...lab manager, research technician, or Principal Investigator. How-to guides for troubleshooting your experiments...believe that science gets better when everyone gets involved. So join the conversation! Do you have a question...