...plasmid cloning blog posts Resources on Addgene.org Find protocols for molecular biology and cloning Read..., as detected by qPCR, but this did not reduce cloning efficiencies.  When compared to fresh columns, ...

...from plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The fourth...

...in your cloning experiments and tired of having to PCR many colonies to find a successful clone? Addgenie...

...co-transform pBLADEONLY. That way, a previously cloned gene of interest, under the pBAD promoter, can ... controlled with light without the need of any cloning.     What advantages does BLADE have over AraC...

...ready-to-use viral preps, pooled libraries, kits, and cloning grade DNA!     This is just the first of many ...

...to COVID-19. We had many protocols for plasmid cloning and working with viral vectors, but what we lacked...

...samples. For a more detailed comparison of these cloning strains, browse the table of common gene mutations...N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia...

...knock-in at a genomic locus or more commonly via cloning of recombinant proteins. Why use a tag? If you...developed in 1985 and is still the most prevalent clone used today! Goat, chicken, mouse, or rabbit? Addgene...

...) (Latynski and Valentovich 2014). We routinely clone the purified amplicons upstream and downstream of...transcriptional readthrough into the MCS to facilitate the cloning of toxic products. To allow manipulation of naturally... they are just spontaneous antibiotic resistant clones. But if they are blue or magenta, they are real...

...protein. These were either (i) coded by the multiple cloning site and therefore consist of random amino acid... often use restriction enzymes in the multiple cloning site for generating fusions. For the POI-fluorescent...PVAT, when we use the AgeI site in the multiple cloning site) is sufficient. For the fluorescent protein-POI...

...monoclonal antibodies are derived from a single B cell clone. One common way of producing monoclonal antibodies...freeze hybridoma cells before culturing or they can clone the genes from the hybridoma into a plasmid to create...

...example: pLXSN has the wild type LTR, an MCS for cloning X gene, an SV40 promoter, and Neomycin selection...either side of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5 kb, but inserts...

...process, you will first need to do some molecular cloning. Depending on whether you are trying to overexpress...downstream experiments Once your gene of interest is cloned into a plasmid, you can send your plasmid to a ...

...template, using Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria are more recombinogenic ...inducer, in this case anhydro-tetracycline (aTc). By cloning in two tandem copies of the gRNA cassette, one ...and the gRNA are Tet-inducible, and a gRNA can be cloned easily into pRH2521 using BbsI. This system results...syringae. These vectors contain BsaI sites for easy cloning of a gRNA of interest. Cytidine and adenine base...

...pre-crRNA arrays simplifies the crRNA cloning process. For cloning, Zetsche et al used four oligos that...

... about a week. Taking advantage of Golden Gate cloning, the Haynes lab’s Protocols.io protocol describes...useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged CRISPR knock-in...

...single cell clones for in vitro CRISPR experiments. The likelihood that a single cell clone derived from...may not be worth the cost and labor when single clones are selected. Conversely, translational research...

...that takes advantage of DNA sequencing to turn mAb clones into expression constructs to be conveniently modified...accuracy while screening. We also compare different clones of the same target to see if the results are the...

...DNA left over for other applications, like PCR, cloning, transfection, or long-term storage. But what about...incomplete. Protein expression strains are not good for cloning Bacterial strains adapted for protein purification...

...website to verify plasmids or validating your new clones, try these tips to make the most of your time and... to help other labs speed up their plasmid and cloning verification steps and free up more time for using...