...the hM4Di, we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold particles...Fluorescent proteins can also be identified using antibodies and immunohistochemistry techniques. Thus, these...

... and antibody labelling. In these experiments, biotin is added to a primary or secondary antibody that...that targets your protein of interest or primary antibody respectively. Avidin bound to a reporter is then... on this complex can bind to your biotinylated antibody. This creates a large meshwork of biotin-avidin...

...going to be looking for recombinant monoclonal antibody plasmids from the James Trimmer Lab published ...distributing 94 plasmids expressing validated antibodies for neuroscience research (see the NeuroMab Collection...specific plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved ...

...protein visualized with a fluorescently-tagged antibody. By employing “marker proteins” which are known... of a cell. This mapping can be done by using antibodies targeted to the marker proteins, however, in ... cell membrane permeabilized in order for the antibodies to reach their intracellular targets. Therefore...

... Cataloging a reagent’s performance as in this antibody ReFigure Analyzing replications as in this ReFigure...ReFigures on the therapeutic use of anti-CD47 antibodies in immuno-oncology. Funding agencies such as ...

...that SpyLigase can be used to link affibodies or antibodies against common tumor markers to subsequently ...approaches to improve CTC capture, but I hope that our antibody tentacles may have an impact on this challenge...proteins by atomic force microscopy (AFM). Maybe polyantibodies could help in the capture of other rare cell...

...transcription factor IRF-1. Immunoprecipitation, using an antibody targeting the 3xFLAG tag, allowed these researchers...well as affinity purification with an anti-Cas9 antibody. These different tag systems and affinity purification...similar affinity purification with an anti-Cas9 antibody, the Li lab found a microRNA (miR483) that binds...

...advantages over their amino acid-based counterparts, antibodies and enzymes, such as: Design and selection ...commercial synthesis (those of us who have used antibodies for diagnostics know how frustrating it can be...hybridization (FISH) where fluorophore-linked antibodies are used to visualize RNA. Low signal-to-noise...

...reagents.  mAb3D atlas validated antibodies: A database of validated antibodies for mouse, human, and marmoset...

...substrates/peptides and antibodies. Example applications of this include coupling to an antibody, whereby excitation...required. These dyes can also be attached to an antibody to detect various targets, such as proteins, nucleic... 568-and Fluorescein Isothiocyanate-conjugated Antibody. Salari, M., Bitounis, D., Bhattacharya, K., Pyrgiotakis...

...e.g. first at 2 months, then at 4 months? Or do antibodies become a prohibitive problem? A: Re-administration...for AAV due to the development of neutralizing antibodies in animal models. All of our engineered AAV are...

...catalogue numbers and manufacturers’ details for antibodies, plasmids (like your Addgene plasmid #), and ...an identifier (a bit like a GenBank ID) to all antibodies, animal models, and software tools in the Materials...

...who have complaints about the unreliability of antibodies. IPI is working to address this issue directly...directly by creating well characterised antibodies against all human extracellular proteins with community-...research tools. BenchSci collates information about antibodies while FPbase curates information about fluorescent...

...viral vectors, and in 2022 we added ready-to-use antibodies. Now, we think of ourselves as a repository for...

...are easily studied in living cells using antibodies. Antibodies could further be used to interfere with...with the identified surface proteins, and such antibodies could thus be interesting drugs. Known drug targets...

...riboprobe binding (via hybridization) specific antibodies that detect the mRNA-riboprobe complex are added...stain the cells that contain this mRNA-riboprobe-antibody complex and, as a result, these cells turn blue...

...scientists produce vital tools in the lab such as antibodies or viruses. If you’re new to tissue culture, ...

...activity (Ni, Titov, & Zhang, 2006), quantifying antibody/antigen interactions (Saraheimo et al., 2013),...synthesized with reagents used to fluorescently label antibodies, and purification is a snap using thin layer ... et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic Concept." PloS ...

...with the most alternative functions – there are antibodies to it and ways to achieve fluorescence with it...Plasmids for Endogenous Gene Tagging in Human Cells Antibodies 101: Epitope Tags  ...

...protein for which there are good flow cytometric antibodies, downstream of the TUJ1 coding sequence. ES cells...types are used. The lack of good flow cytometric antibodies for many CNS-resident cell types (microglia are...