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Showing: 101 - 120 of 285 results

  1. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...in the correct orientation. These plasmids use a novel synthetic RPBSA promoter, which is made up of a ...PubMed. 2. Mates L, et al. Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables ...

  2. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated...retrovirus) while others are non-integrating (adenovirus and AAV) but can still express episomally long-term...

  3. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...10,000 users per month in November 2014 to ~30,000 users per month in November 2015. We will continue ...

  4. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...experiments are crucial in demonstrating the true innovation, impact and, in turn, the big picture of the ...committee is not convinced that your project is an innovative solution with a strong impact, or a journalist...

  5. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...perform proof-of-concept experiments for discovering novel therapeutics. Dave is a chemical biologist with ... everyone but these projects could also lead to novel yeast creations that may not be pursued in an academic...

  6. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...contributed by Mary Tamer from BioBuilder, an innovative nonprofit bringing the study of synthetic biology...state-of-the-art lab embedded in LabCentral’s community of innovators that opened in August 2017.  This flexible teaching...

  8. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...new resource from Jennifer Doudna’s lab at the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA...CRISPR Beyond SpCas9 More resources from the Innovative Genomics Institute      CRISPRpedia IGI’s ...

  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...powerful tool in almost all disciplines. In biology, innovative new imaging approaches are constantly being developed...and disciplinary boundaries and can allow for innovative organizations and companies to provide problem-solving...

  10. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...recently in a paper published in F1000Research on November 2016. Due to the nature of the postdoc position...been made publicly available (below). However, on November 22nd a preliminary injunction against the FLSA...

  11. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...proteins as bait has limited utility for identifying novel RNA-binding proteins that interact with an RNA of... applications of RaPID: Discovery. To discover novel RNA-protein interactions, Ramanathan et al. designed...

  12. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... workflow and its analysis can be found here: A Novel Next-Generation Sequencing and Analysis Platform...Hanley L, Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation Sequencing and Analysis Platform...

  13. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...through your proposal, ask yourself: is it clear how novel my findings will be and why these findings will ...problem can allow you to approach that problem in a novel way. Finally, engineering new applications to solve...

  14. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions for storage...Resources on the Addgene Blog Learn All about Adenovirus Associated Virus New to Virus? Check out Our ...

  15. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...support from the Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative Cell Census...Atlas will foster collaboration, progress, and innovation by allowing scientists to find and utilize reliable...

  16. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...recombinant amino-terminal acetylation system and novel in vivo high-throughput screen for molecules affecting... Vivo Imaging of Protein-Protein Interactions. Filonov GS, Verkhusha VV. Chem Biol. 2013 Vladislav Verkhusha...

  17. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    .... https://doi.org/10.1074/jbc.RA120.01289     Novel protein cage provides simple enzyme protection in...Gabrielle Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting proteins in yeast...

  18. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...When contamination is suspected, we proceed to de novo assembly of the unmapped reads, employing our bioinformatics...Hanley L, Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation Sequencing and Analysis Platform...

  19. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...Update (November 18, 2016): Researchers from a variety of institutions recently reported their inability...surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can form a complex with...

  20. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...) system functions by harnessing the power of a novel degrader of the 12 kDa protein FKBP12F36V. By fusing...can be degraded by this selective degrader. The novel degrader, dTAG13, is a small molecule which induces...
Showing: 101 - 120 of 285 results