...showed that it can alter motor responses in C. elegans. Find the split BoTN/B plasmids at Addgene. Liu...in mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved...

..., if you are studying a protein that may bind a ligand during your experiment, then you’d want to make...antibody that recognizes an epitope far from the ligand binding site (Mould et al., 2016). If your antibody...part of your protein that may be masked while the ligand is bound, then you’ll get a false negative signal...Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced Epitope Masking. Journal of Biological ...

... of informatics. I am a molecular biologist who began coding for my thesis project a few years ago when...language favored by biologists. Almost as soon as I began, I realized that I needed a system to keep track... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software You Can Use to Save Time ...

...your current organization but also important to build relationships outside your organization. The mentor...what makes a good mentoring program. If your organization has a mentoring program, consider tapping into...upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking someone...

...their CV or grant applications. A number of organizations (such as ASAPbio) advocate for preprints, and...manuscript to a journal for formal peer review. Some organizations, including some journals, also support open ...general public (say, policymakers or community organizations) to use your work but don’t think they’ll have...here at Addgene! Along with other open-science organizations, we support scientists in sharing their results...

...integrins (cell-adhesion proteins) targeting different organs. This means that exosomes not only promote microenvironment...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain miRNA...angiogenesis (Witz et al., 2009). Then, scientists began focusing on the extracellular matrix and fibroblasts..., et al. "Tumour exosome integrins determine organotropic metastasis." Nature 527.7578 (2015): 329. PubMed...

...can take to verify your own plasmids. Staying organized With hundreds of samples coming through our doors...first step to ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid...selection markers. You don’t need a fancy database to organize your own plasmid collection, although you will...found here. Finally, you will want to have a well-organized database of expected sequences, either for the...

...monitoring and analyzing autophagy of cytosol and organelles in yeast cells. Autophagy (Greek for “self-eating...by a lack of nutrients and targets cytosol and organelles to the vacuole/lysosome for degradation and recycling...more fine-grained detail about what proteins or organelles are being degraded) and it is slow. Alkaline...for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy,4(2), 205-213. doi:10.4161...

... a multi-gene pathway to study and a reference organism where deletion strains are available. Then, staff...clone similar sequences from a less-well studied organism into overexpression plasmids. Students next transform...transform deletion strains from the reference organisms with the overexpression plasmids. Finally, students...Saccharomyces. cerevisiae, makes a great reference organism for the project because it is non-pathogenic, ...

... time to read them. Organizations: Accounts for nonprofits, research organizations, and universities often...hashtags to library entries, which can help you organize and search for papers later. Pro tip Learn more...search terms will show up in your feed. Feeds are organized by papers published each day, week, or month. ...

...interactions New auxin inducible degron system for C. elegans New CRISPR plasmids New items from the viral service...Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly of multiple... the auxin inducible degron (AID) system in C. elegans By Alyssa Cecchetelli The auxin inducible degron...degron (AID) system has been extensively used in C. elegans since 2015 to conditionally degrade proteins of...

...culture that will hopefully help you keep a well organized lab and contamination free environment for successful...should keep in mind that UV light only kills microorganisms that it strikes directly. UV light exposure...consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin)! It’s ..."Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis, and gene expression disregulation...

...there were a variety of ways to monitor cell, organelle, and protein localization. For instance, you might...microscope, fractionate samples to isolate particular organelles and their contents, or perform in situ hybridization...predictable kinetics over 14 hours of expression in C. elegans embryos. However, this initial timer was a tetramer...could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus embryos. Of course, timers may ...

...In April 2022, physicians in Scotland began noticing unusual cases of acute hepatitis in the pediatric... amassed in places around the world, hypotheses began to develop that this illness was caused by either....73.8.6930-6936.1999. PMID: 10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & ...00379727-84-20714. PMID: 13134217. Schmitz, H., Wigand, R., & Heinrich, W. (1983). Worldwide epidemiology...

...grow liver stem cells and liver-like mini-organs or organoids, neither of these can substitute for a liver...talk preparation: Define your research vision. Organize your vision into a chalk talk format. Practice...questions from open ended screens and data collection. Organize your research vision into the chalk talk format...

... Fig: 2: Expressing mARGAna in MDA-MB-231 cells. (a) Plasmid diagram of mARGAna. (b) Schematic of the...the general method to create your own mARGAna cell line. (c) Z-projected phase contrast images of GV expressing...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature 553, 86–90 (2018...

...Awareness Organization (ADAO) are working toward such a ban, and the president of the organization, Linda... has spread from where it originated to another organ in the body. Such misdiagnosis and late discovery...tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the surgeon ...

...Nonetheless, the initial release caused a storm as the organization left open the question of how much data was ...to make the underlying data easily available, organized in a form that can be understood by others, balanced...each individual video. To PLOS’s credit, the organization subsequently clarified what the policy intent...researchers. Yet accurate and comprehensible organization of data is essential for efficient science. ...

... using a viral approach Genetically modified organisms like transgenic mice are important tools for understanding...specific to interneurons in mice as well as other organisms, opening the possibility of using these tools ...protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1), some of the exciting... a stable monomeric form as determined by the organized smooth endoplasmic reticulum (OSER) assay. A major...coselection, analogous to methods previously used in C. elegans, targets the sodium/potassium pump ATP1A1 in addition...

...our days more effective, and our schedules more organized. From the alarm in the morning, to the daily news...note-taking tools, others using excel sheets or organizing folders in complex ways, there are solutions ...institution, the transition is smooth as all data is organized within an ELN already. Investing some time in ...makes it much easier for lab members to manage and organize their work at every step of the research process...