... of low-toxicity RNA editing tools.   Fig. 1 Schematic of luciferase RNA knockdown assays using...PRIME, PASTE relies on single-stranded nicks instead of the double-stranded breaks used in other insertion...target. In addition, when they looked at the transformed E. coli expressing Cas7-11, they found no evidence...broadly similar to shRNA and Cas13 systems in a range of mammalian cell lines. However, in those same ... of landing sites via Cas9-directed reverse transcriptases, followed bylanding site recognition and integration...

...processed since the declaration, only a small fraction (<1%) of authors have asked for advice about the scope...supporting datasets must be logically annotated and arranged, as in an actual publication and not require interaction...responsible for storage.  If datasets are too large to transfer to a journal repository, they are likely too large...albeit unintentional, tendency to work against transparency. This opens science to charges of elitism and...opportunities for research as well as increasing transparency. Thank you to our guest blogger! Jim Woodgett...Will Pay for Public Access to Research Data? - by Francine Berman and Vint Cerf (Science, August 2013) PLOS...

...fun together! Laura’s Top 10 Event Planning Tips! 1. Take suggestions Some of Addgene’s best events have... elaborately pranking each other, dressing in costume, and holding competitions (ranging from cook-offs...orderly and timely fashion. 9. Ask for help My grandma used the old adage “many hands make light work”..., evite, posters, megaphone, or your company’s intranet. Don’t forget about the power of simple word of...

...antibody bonding strengths.    . Steps of an IP: 1) Protein extracts from cells or tissues contain a ... protein of interest, such as a sample transiently transfected or stably infected with the protein of ...downregulated, examine a protein’s stability or post-translational modifications, or study how a target protein...experiments using a tagged protein may not be translatable to the endogenous system and will need to be...

...References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone...popular papers, but looked for papers from a wide range of authors. Just start writing When I first started...drafts of the manuscript. This is the time to transform the document into something cohesive-- change ...Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our...

... reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image from...loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a colorimetric readout that can... 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase constructs from...toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step...

...two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze...). But is it possible to bring the kinds of transformative educational experiences afforded to those fortunate... now connects MDH-focused CUREs at 50 schools, ranging from R1 institutions to community colleges, and...NADH production.   CUREs are beginning to transform undergraduate education in the sciences by making...

...commandments for making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are ...responsibilities. Another tip: don’t miss your grandfather’s 100th birthday, as your mom will never forgive...when you can’t get home, babysitting or running errands. Give help when you have time, and in return you...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments...

...for your first journal club to begin! Figure 1: Discussing next-generation sequencing at the Addgene...of current new and exciting literature. As I transitioned out of academia and away from the bench I certainly...

...can make this fun activity relate to STEM.” Figure 1 shows the activities we had for my school’s Math &...olds in a single evening. Grades K-5 cover a broad range of attention spans, skill levels, and STEM exposure...aspects of talking to 8th graders was the broad range of questions I received. I gave my presentation ...