... work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ...various plasmids for overexpression and knockdown. Addgene also has a number of insect vectors to consider...easy way to see what is available is to browse Addgene’s Collection under the insect expression system ...species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly ... survival. For those interested in development, organs of interest, such as the intestines, can be dissected...have!) (4). The power of Drosophila as a model organism is in its amenability to genetic manipulation....PMCID: PMC3172133. Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read ...

... al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore the phenomenon...and optogenetic tools exist, humans (like many organisms) are opaque, which makes most biomedical applications...There is a variety of research on whether and how organisms respond to magnetic fields (Karki et al., 2021...sometimes seen controversy, and the interactions of organic molecules like proteins with magnetic fields generally...magnetic responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011...doi.org/10.1038/nn.4265.  Additional resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids...

... can function to break membranes of different organisms. And finally, we demonstrated that R bodies can... wild type and mutant R bodies can be found at Addgene here. What can R bodies do for you? R bodies are...speculatively, R bodies might endow divergent organisms with new functions. For inspiration, we can look...Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization and expression of genetic determinants for synthesis...26814170. Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., ...Central PMCID: PMC26518. Additional Resources on the Addgene Blog Read About Other Hot Plasmid Technologies...Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids...

... Biology Resources on the Addgene Blog Deposit your plasmids with Addgene and make your work more accessible...our days more effective, and our schedules more organized. From the alarm in the morning, to the daily news...note-taking tools, others using excel sheets or organizing folders in complex ways, there are solutions ...institution, the transition is smooth as all data is organized within an ELN already. Investing some time in ...makes it much easier for lab members to manage and organize their work at every step of the research process...Twitter @scinoteELN Additional Resources on the Addgene Blog Learn about more tools for reproducibility...

...scientists to edit the genome of virtually any organisms on this planet, CRISPR-Cas systems were merely... to quickly detect the presence of infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing...ID PMID: 30337455. Additional resources on the Addgene Blog Learn more about DETECTR Browse all of the...Download our CRISPR cheat sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR...

...’s team devised the plasmids now available in Addgene’s repository for use in neurons, both in culture...tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt to influences...use is by no means limited to particular model organisms or cell types. “This tool is really flexible and... Kendall Morgan...

...stakeholders, the product managers collate and organize that information and form it into a roadmap--a... features, programs, or tools to build. After organizing the information, a software product manager works...everything running smoothly, you’ll want to be really organized. Often, product managers will have three or more...twitter @rgrubinstein. Additional resources on the Addgene blog Discover more science career options Check...

...This is the fourth post in the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot...like to start a group mentoring program at your organization, contact me! I can provide a step-by-step, how-to... people from different labs, departments or organizations as it brings a diversity of perspective. Sometimes...transition. Schedule your first meeting and get organized. Discuss your general areas of interest and what...

...sharing Addgene is all about helping scientists share between labs, and people work hard at Addgene to make...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ...sports, movies, and just about anything. As our organization grew, we continued this tradition. We now have...as one of their favorite parts about working at Addgene. We’re a small non-profit, so we can only provide...delicious) team building activity that is easy to organize. We’ll dedicate a post to these competitions in... started Hack Days. During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than...Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional teams...

...author do not represent a formal stance taken by Addgene or its staff. In Washington, D.C. as well as sister...rights (2). Less formally, governments are the organizations that make and enforce the rules of the lands...climate change, the safety of genetically modified organisms (GMOs), the absence of a link between vaccines... while their website lists over 200 partner organizations, and 514 satellite marches are scheduled to ...author do not represent a formal stance taken by Addgene or its staff. Many thanks to our guest blogger,...Chronicle of Higher Education: “March for Science” Organizer Says It’s About the Public, Not the Scientists...

...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed ...systems The ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015). “A Scalable...26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and ...57. PMID: 26701308. Additional Resources on the Addgene Blog Brush up on Homology Directed Repair and ...

...academia and industry. As a result over 90% of Addgene’s COVID-19 resources are now available to scientists...these groups were willing and able to listen to Addgene’s new approach of working with the industry-based...in the background became a normal occurrence as Addgene and our university partners quickly assessed how... happy to have the support of the following organizations to share materials more broadly to accelerate...allowed them to synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us...COVID-19 plasmid collection Beyond COVID-19: Addgene’s plasmid collections available to industry scientists...to industry? Over the past 8 years, several organizations have made their plasmids available beyond academia...

...touch! Resources at Addgene Read Our Posts on Networking Get Career Advice from Addgene Bloggers Learn How...and some of the great biological societies that Addgene has had the opportunity to work with at Freshers...support each other and provide advice. The society organizes numerous recreational and educational events throughout...outside academia. Inspiring future scientists At Addgene, we’re incredibly glad to see these biological ...society or event which would benefit from some Addgene support? Please get in touch, as we aim to support... How to Get Involved in a Profession Organization ...Cambridge and within the general public. The society organises talks by prominent scientists, including Nobel...

...that expound on bench skills and experience. Most Addgene PhD level job descriptions clearly describe that...send directly from a mutual connection. If the organization is small, any person at the company might be...be able to help you. If the organization is large, you will need to be more directed and target someone....  You might also enjoy the other posts in the Addgene Blog Scientist Careers Series.    ...

... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources...many cases, however, a GFP-tagged cell line or organism has already been made, and it would be expensive...already have created a transgenic GFP-containing organism,” says Hanson, “using a gene encoding one of the... GFP is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used...PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis...

...Resources at Addgene.org Find careers at Addgene Watch out career videos Sign up for the Addgene quarterly...making this transition. In this installment of the Addgene career guide, we’ll think about what causes this...job candidate for a senior scientist position at Addgene, the skeptical applicant asked if she would partake...of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list of things... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is always ...someone who coordinates cross-team projects at Addgene, I find that my vocabulary improves on a weekly...

... a Professional Organization Resources on Addgene.org Check out Career's at Addgene ... the Management Team that steers the organization. As an Addgene vet familiar with the expertise and skills...joined Addgene’s Green Team, a like-minded group of co-workers who want to make our organization as environmentally...unless we manage to make Addgene the most environmentally friendly organization on the planet, in which...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting... by some colleagues as the “cross-team guy” at Addgene, an unofficial title that I happily embrace (and...possibly understand every nuance of how kits work at Addgene, but with the help of these people and others, ...

...facing a financial crisis. As a nonprofit organization, Addgene charges enough for our services to run our...funding organizations to request support for our operations until our normal work resumed. Addgene distributes...materials via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small... all over.” And we sent the Addgenies home.  At the same time, as Addgene started dealing with this new...resource for scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure...science sharing as we can get. The effects of Addgene’s brief shut-down and the subsequent fluctuations...don’t have space in this blog to call out every Addgene Team because they’re all doing amazing work during...

... Stopwatch! Any organization that deposits with and/or requests materials from Addgene can qualify. We...between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene Master Reception...between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA: material ...question? Refer them to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the...commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology Transfer...arrival of the newest plasmid you requested from Addgene...the plasmid that will help you run that last ... for depositor and requestor alike. That’s why Addgene works closely with TTOs to help protect our depositors...

...FOP until a cure is available. Addgene and AJ's Avengers This year Addgene, where my wife Lianna has made...their plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the ...years. Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers... Disease that runs on behalf of the National Organization for Rare Disorders (NORD). NORD raises money...helping us fight against FOP in two ways. First, Addgene is distributing ACVR1 Plasmids from a variety of...We will be collecting donations for IFOPA, an organization dedicated to raising funds and awareness of ...Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease...