Technical Design of a Western Blot
Type
Blog Post
Published
Aug. 22, 2024, 1:15 p.m.
...western blot. Polyclonal antibodies, which bind to multiple sites on an antigen, are cheap and can help amplify...sequencing. While rAbs are monoclonal, recombinant multiclonal antibodies — mixtures of different rAbs that ... likely higher background noise as well, and multiplexing is challenging and limited.
Direct westerns ...sturdy membrane that can be stripped and reprobed multiple times. It doesn’t degrade during long-term storage...of protein.
These enzymes cannot be used for multiplexing, and the solutions used to provide the chemilumescence...Fluorescent proteins, while more expensive, allow for multiplexing. They have a ten-fold greater dynamic range ...limited dynamic range, which means it may take multiple exposures for a blot with varying signals on it...