Plasmids 101: Inducible Promoters
Type
Blog Post
Published
Jan. 18, 2018, 2:34 p.m.
...How are inducible promoters regulated?
Inducible promoters can be regulated by positive or negative control...Addgene!
Chemically inducible promoters
Chemically regulated promoters are among the most common inducible ...protein purification. When arabinose is absent, regulatory protein AraC binds O and I1 sites upstream of...complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites. Supplementing...include positive inducible alcohol and steroid regulated promoters commonly used in plant research.
...Example
Activator
Addgene Plasmid/Kit
Alcohol-regulated
AlcA promoter
AlcR
pGGA008 (AlcA) and pGGC011...AlcR) from red flame GreenGate kit
Steroid-regulated
LexA promoter
XVE (synthetic)
pFZ19 (yellow flame...