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Addgene
Showing: 81 - 100 of 225 results
  1. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... old-fashioned at all. With the development of new fluorescent pairs and the emergence of faster measurement...
  2. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ..., a method called pyrosequencing. The drive for faster, higher throughput and less expensive sequencing...both time and research progress. These will bring faster, higher throughput, and potentially more accurate...
  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ... later became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR...ligands (Wilhelm et al., 2021). While HaloTag reacts faster for some dyes, others, especially some nonfluorescent...
  4. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Aug. 6, 2020, 2:30 p.m.
    ...generate ABE8e(TadA-8e V106W), which edits ~590-fold faster than the TadA from ABE7.10 without increasing off-target...off-target editing Richter et al., 2020 ABE8 590x faster adenine base editing than ABE7.10 Gaudelli et...
  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...reprogramming period and the plasmid will get diluted faster when cells are actively dividing. The typical drawbacks...less time to complete. Morphology is a simple and fast to evaluate. iPSCs should form compact colonies ...
  6. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...actuator pair. Ideally, indicators should possess fast voltage sensing kinetics and have no spectral overlap...named CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator...visualize the Broccoli-tagged RNA. This technique is faster and less labor intensive than northern blotting...
  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...small protein is over-transferring, try using the faster semi-dry transfer, which works better for small...low levels of proteins. In this case, it might be faster to run the same amount of protein in every lane...
  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...Chronos, is blue and green light-excitable and has faster kinetics than those of other channelrhodopsins....optimizing protein expression and purification in a fast, efficient and affordable way. Scholz et al.., BMC...
  9. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol...
  10. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...data on plasmid deposits and distirbution for these fast moving fields.  Addgene is a global nonprofit plasmid...
  11. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ... cannot be washed or reused/repurposed in some fashion. Addgenies swapping out printer cartridges can ...
  12. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...CRISPR material for you!  The CRISPR field moves fast. That’s why we’re bringing you a brand new third...
Showing: 81 - 100 of 225 results