Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
Type
Blog Post
Published
Sept. 3, 2019, 1:15 p.m.
... versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that ...Expanding the targeting range of CRISPRCas9 by engineering SpCas9
Article contributed by Shreya Vedantam...editing. PAM sequences can be from 2-6 base pairs in length and follow the DNA sequence targeted by the Cas9...PAM to recognize the target. Osamu Nureki’s lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for...recognizes NG rather than NGG. In addition to the engineered SpCas9, they also fused the SpCas9-NG with the... promoters and enhancers, or have attempted to engineer loops to create genomic interactions that wouldn...