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Showing: 81 - 100 of 254 results

  1. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...serious adverse effects, and their immunogenic or carcinogenic profiles have limited their usability in clinical...

  3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...” column. Relevant Mutations Indicate the amino acid change as well as any functional consequences...hygromycin, kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of...

  4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ... in N-terminal fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput...Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et al. Improved split fluorescent proteins ...

  6. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...for sequencing, we feel confident that these few minor mismatches usually don’t affect the function of ..., we check to see if they affect the translated amino acid. We also confirm that the species of the gene...

  7. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...Nature chemical biology. 2021 May;17(5):509-18. Inoue M. Genetically encoded calcium indicators to probe...Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino M. Genetically encoded fluorescent indicators for...

  8. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...farmers markets), and serious ideas (how to stop rhino horn poaching using biotechnology) were thrown on...Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted ...

  9. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems are ideal for... to assemble proteins with labelled or modified amino acids that are useful in different downstream applications...

  10. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...often doubles the volume of culture used for inoculation if we get inadequate plasmid DNA yields. Why ...growth conditions, and the material used for inoculation. To further complicate matters, plasmid copy ...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...discovered in picornaviruses, are short (about 20 amino acids) and produce equimolar levels of mulitple ... type or experimental conditions.   Peptide  Amino acid sequence* T2A: (GSG) E G R G S L L T C G ...

  13. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...(ADAR). Such a system would convert adenine to inosine, which the translational machinery treats like ... ADAR1 and ADAR2, which deaminate adenosine to inosine in RNA, creating a functional A->G change. They...

  14. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ... then be recognized by a DNA-binding molecule (Hoshino and Fujii, 2009). Other forms of enChIP, that do...PMID: 23849981. PubMed Central PMCID: PMC3770145. Hoshino, Akemi, and Hodaka Fujii. "Insertional chromatin...

  15. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...of culture you are using and whether you are inoculating from a frozen stock or a colony from an LB plate...plate. In my hands, a 5 mL starter culture inoculated from a colony on a plate will reach an optimal density...

  16. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance genes in E. coli using plasmid and ...plasmid. In the case of the genomically encoded quinolone resistance, mediated by a single base pair change...

  17. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...affinity tag, generally a relatively small sequence of amino acids, is basically a molecular leash for your protein...; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability At N-terminus, promotes...

  18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea...formed from a maturation reaction of three specific amino acids at the center of the protein (Ser65, Tyr66...

  19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...packaged into adeno-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter jejuni ...editing than SpCas9 (Kim et al., 2016).  The Acidaminococcus sp. BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM...

  20. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F (2016...Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (2015) Discovery and Functional...
Showing: 81 - 100 of 254 results