...repair and genome engineering CRISPR-Cas9 has revolutionized the genome engineering world and made targeted...immediately applicable in DSB-induced genome engineering, it has biological importance for the repair ...CRISPR forum moderated by the Zhang lab CRISPR Challenges: Standardization and HDR CrispyCrunch Optimizing... (2018). Pubmed. CRISPR References: Genome engineering using the CRISPR-Cas9 system. Ran FA, et al. ...546; (2015). PubMed. Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System. Li, K...PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed. Enhancing..., et al. Poster at Keystone Precision Genome Engineering. 2017.  Easi-CRISPR: a robust method for one-...

...or intentional release of engineered organisms. “Is [the organism] engineered? Where did it come from? ...reached out to MIT labs in the Synthetic Biology Engineering Research Consortium. That is how Aditya Kunjapur...organism in the lab. Predicting lab-of-origin of engineered DNA This isn’t the only time researchers have...plasmid datasets) to predict the lab-of-origin of engineered DNA. Both of these groups have generated robust...complementary tools in a time where biological engineering and synthetic biology is expanding rapidly.  ...Deep learning to predict the lab-of-origin of engineered DNA." Nature communications 9.1 (2018): 3135....

...theoretically, at least) fully and freely engage or disengage at will.  Informal education is education...typically ad hoc, for learners who can choose to engage or disengage with a very high level of independence, such...than average and are more likely to spend time engaging with science communication efforts.  Scientific...however, many others partner with formal learning to engage students at all levels.      Jargon is field-specific...space for people outside of the primary audience to engage if they so wish. (And let’s be honest: are you ...

...summarizes Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional Programs with...However, these approaches are often met with the challenges of regulatory bottlenecks and their scope is ... ‘biofactories’, most successes in metabolic engineering have been limited to the overexpression of various...host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and E. coli. The utility...scRNA design is readily apparent in metabolic engineering. The authors elegantly demonstrated the potential...By no means limited to the field of metabolic engineering, this is such a powerful and flexible technique... 1173-1183. (2013). PubMed. Zalatan, J. G. Engineering Complex Synthetic Transcriptional Programs with...

... versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that ...Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam...editing. PAM sequences can be from 2-6 base pairs in length and follow the DNA sequence targeted by the Cas9...PAM to recognize the target. Osamu Nureki’s lab engineered SpCas9 (SpCas9-NG) with relaxed preferences for...recognizes NG rather than NGG. In addition to the engineered SpCas9, they also fused the SpCas9-NG with the... promoters and enhancers, or have attempted to engineer loops to create genomic interactions that wouldn...

....  The question for her then was this: Can you engineer CRISPR-Cas9 activators that work well enough on...that they could be used by people with little bioengineering expertise? “This is the next step in high-throughput...Nature in December, Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they...Synergistic Activation Mediator or SAM. SAM is an engineered protein complex for the transcriptional activation..., unlike the mouse, we can’t rely on genetic engineering to optimize humans for CRISPRi and CRISPRa.” ...activating any human gene, go to http://sam.genome-engineering.org/. Crystal structure of Cas9 in complex with...Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD,...

... explore their Local Engagement Playbook for step-by-step guides on how to engage in your local community...That’s what makes it so wonderful. The most challenging part of jumping into this career path from academia...ranges from astronomy to bioethics to chemical engineering and everything in between. Plus, there are many...folks is on Twitter- start an account and start engaging with the #scipol hashtag. Often there will be ...advocacy as a supplement to your career through engagement with your local or state governments. Most communities... your hair out, your successes, failures, and challenges you’ve overcome. This information may help you...completely new field without issue. This may be more challenging if you are trying to apply for an outright job...

...would work with our research team and software engineers to update our Lab Inventory Management System ...notice something: even though our scientists and engineers were attending the same meetings and hearing the...always knew from context what a “plate” was. Our engineers thought they knew.  As long as you have context...of “plates” (Fig. 1). When you have to tell an engineer that you want the LIMS software to create a barcode...meetings and 2) creating a pictorial guide for our engineers (Fig. 2). Creating this guide was relatively easy...not have to communicate with a team of software engineers in your lab, you likely will need to write protocols... convey the same concept to Morgan, our senior engineer? The answer to these questions--which were real...

...Sainsbury Lab website or Engler & Marillonet. Golden Gate and genome engineering In early 2011, the Bogdanove...within both the synthetic biology and genome engineering fields. We’ll walk you through how to apply this...ligation process is close to 100% efficient. Another strength of Golden Gate cloning is its scalability. Unique...overhangs to be efficiently assembled. Scientists can engineer unique enzyme recognition sites that flank their...cloning method with high throughput capability. Engler C, Kandzia R, Marillonnet S. PLoS One 2008;3(11...Epub 2011 Apr 14. PubMed.   Golden Gate cloning. Engler C, Marillonnet S. Methods Mol Biol. 2014;1116:119...

... genetic engineering, with the ultimate objective of genome design.” It’s genetic engineering on a genomic...needed for anyone to begin considering genetic engineering projects in a very big way. In one of his latest... with Cas9 guided by different promoters. The challenge in building genetic circuits is getting enough...scale could be created. Someone could take on an engineering project including hundreds of genes and regulation...complete toolbox for taking on very large genetic engineering projects. Addgene: How easy is it to do this ...animals make. That will be an outcome of genetic engineering.   Thank you to Chris Voigt for speaking with...

...elegans Fluorescent timers for studying cell cycle length The CRISPR corner New from the viral service ...synaptic connections and physiology is particularly challenging as they are great in number, generally very small...come together, they fuse and fluoresce. The team engineered C. elegans cell lines to express wrmScarlet1-... small wrmScarlet11 which is less than 60 nt in length to tag the target protein. The team validated this...10.1101/2020.07.02.185249 Predicting cell cycle length with a histone-fused fluorescent timer By Kate ...fusion protein can be used to determine cell cycle length by examining the ratio of blue to red cells. Cells...plasmids at Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a ...

...point: Test different lengths of the primer binding site, starting with a length of about 13 nt (Anzalone...optimized (Anzalone et al., 2019). Test different lengths of reverse transcriptase template, starting with...templates, it is even more important to test different lengths and sequences, as unintended secondary structures...Anzalone et al., 2019). Figure 2: Starting lengths for optimizing your pegRNA primer binding site ...Editing the PAM prevents the prime editor from re-engaging with DNA it has already edited. Created with BioRender.com...., Chen, J. C., Hsu, A., & Liu, D. R. (2022). Engineered pegRNAs improve prime editing efficiency. Nature...

...(7629):452. doi:10.1038/nature20149 Jiang Q, Meng X, Meng L, et al. Small indels induced by CRISPR/Cas9...RNAs (lncRNA). lncRNAs are over 200 nucleotides in length and lack an open reading frame, so small deletions...promoter and enhancer sequences remain intact (Engreitz et al. 2019). The interactions between ncRNAs ...RNA-targeting CRISPR enzymes for transcriptome engineering References Jinek M, Chylinski K, Fonfara I, Hauer...18(12):2097. doi:10.1080/15476286.2021.1899500 Engreitz JM, Haines JE, Perez EM, et al. Local regulation...in Plants with the CRISPR-Cas Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. ...

... Centre for Public Engagement (NCCPE) provides on-site courses in public engagement for institutions and...science blogs like @WIRED Science. Don’t be scared to engage with them. Comment on their activities and participate...using social media as a scientist here. 2) Public engagement - Participate in science festivals and outreach...talking in public, volunteer at science festivals. Or engage with non-scientists in an unusual setting: Discuss...by advertising your blog on social media and by engaging with other science bloggers. If you don’t have...guest blogger, Sarah Schmidt! Sarah Schmidt is engineering resistance against Fusarium wilt disease in bananas...

...since the Future of Research Symposia Ongoing challenges facing the scientific enterprise and the way ...revealed that young scientists are aware of, and are engaged in, these issues which will continue to shape the...landscape of the scientific enterprise will provide challenges and opportunities for us all.  For example, implementation...categorize all postdocs at institutions, will be challenging. At the same time, there is much opportunity ...the pressure on for further increases will be challenging, but will be aided by the development and implementation...science funding and funding mechanisms require an engaged sector with effective advocacy skills. Advocating... is an important way to push the balance from challenge to opportunity. We hope to see you at the upcoming...

...of Revolution Bioengineering.  Last year we started a company. Revolution Bioengineering is two responsible... We both had an applied science bent, so the engineering aspect of this job appealed to us. However, since... publish or attend conferences makes it very challenging to compete in today’s tough academic or industry...team. We have confidence that we can take on any challenge and quickly become experts in something, even ...comment.  In our day job, we worked on plants engineered to detect explosives and rapidly indicate their...careers: to build something new and beautiful, to engage with the public in a meaningful way about science...

...xkcd.com Science communication should be fun and engaging for the communicator as well as the audience, ...enjoy and that plays at least slightly to your strengths. Otherwise, you’re going to drop your project ...graphics for visuals. However, it’s much more challenging to design a hands-on interactive activity using... Small tidbits and fast/fun facts are easy and engaging on social media, but deep dives are often more...complexity. Podcasts Medium Easy for audience to engage while multitasking. Easy to host guests remotely...etc… Low (except for space) Real-time audience engagement and response. High connection levels and interaction...geographic location) Opportunity for real time engagement while allowing for recording/future access. Remote...

...those engineered DNA-binding molecules for the first time and named the new technology as engineered DNA-binding...insertional chromatin immunoprecipitation (iChIP) and engineered DNA-binding molecule-mediated chromatin immunoprecipitation... locus, which is the rate-limiting step. When engineered DNA-binding molecule technologies such as TAL...However, it struck me that direct use of such engineered DNA-binding molecules even enables us to skip...Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation... and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...

...studying chemical engineering. Mengtong "Tom" Duan is a PhD student studying bioengineering. They are both...agents. It ended up being very straightforward to engineer various cell lines from different tissues and ...transposase. In rtTA-cell lines, it is better to first engineer the cell line with rtTA, then integrate the mARGs...was sufficient to saturate expression. When engineering a cell line, cotransfect the mARG cassettes 2...For in vivo experiments, simply induce injected engineered cells via regular intraperitoneal doxycycline...

...solutions to complex challenges. As a cross-disciplinary researcher, the challenge of keeping up on studies... Sharing your research with the world can be challenging. After months, or years, of grueling effort to...biology or international relations.  Many of the challenges in science today are too complex for any single...looking for funding, or are by researchers for whom English is a second or third language (making publishing...publishing their research all the more challenging even when it is of quality). On the site you can, for example...Vocalization. The video summarizes the research of Drs. Feng and Bass published in a recent issue of the open...