Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
Type
Blog Post
Published
June 28, 2018, 3:01 p.m.
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Derive clonal lines by...intended target site. With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field...delivery
Epitope tagging of mammalian genes using CRISPR depends on successful delivery of three reagents...using custom synthetic two-part cr/tracrRNAs for CRISPR experiments (in collaboration with Integrated DNA...tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were pre-assembled...csRNP knock-in experiments
If your lab is new to CRISPR/Cas9 experiments, there is no need to worry at ...