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  1. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...genomes. Unlike plasmids used for expression in yeast and bacteria, plasmids used for stable transfections...

  3. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...Gate Assembly. They built this toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-...

  7. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...strategies are not available to those working with yeast or plants. Using MoClo, or better yet, a pre-made...

  9. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...efficiently and reversibly bias inheritance in wild yeast. bioRxiv 013896 (2015). doi:10.1101/013896. bioRxiv...methods are easy to implement and have been tested in yeast5. Ecological confinement involves performing experiments...

  10. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous recombination...

  12. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...microscope will help you identify bacterial and yeast contaminants. Set up a routine to split cells Don...

  13. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...It does work in mammalian cells, mice, and even yeast, though (we have a kit!). Choosing a degron tag ...

  17. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...specific secondary structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms...
Showing: 61 - 80 of 95 results