...The library was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses...that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides against...obstacle. With CRISPR-X, dCas9 is used to recruit an sgRNA with MS2 hairpin binding sites, which then recruit...

...Shlyueva D, Müller I, Helin K (2016) Optimizing sgRNA position markedly improves the efficiency of CRISPR...

...Root DE (2014) Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation. Nat...

...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...

...28414855. 2. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target...

...depending on perspective – to work with inefficient sgRNAs in my initial direct screening efforts, which were...

...Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.” Genetics 200(2) (2015): 431-441. PubMed PMID:...Assembly. Using BsaI, gRNAs are cloned into one of 12 pYLsGRNA plasmids, which contain various promoters and ...

...sequence which could be adjusted by changing the sgRNA length. The system could be further refined by the...

... a single, unique, and constitutively expressed sgRNA targeting the 5’ end of each essential gene. Koo...