...educating the public and organizing events. I contacted local professional organizations and nonprofits. I volunteered...Central Indiana Science Outreach, or CINSO. We organize fun science events for adults and professional...communication, editing, marketing, and website organization. My short experience with my blog helped immensely...establishments such as museums, professional organizations, and other nonprofits to gain support for CINSO...more people to quit the lab. Resources on the Addgene Blog Read Joanne Kamen's Blog Series on Mentoring...Browse All of Our Career Posts Resources on the Addgene.org Find Plasmids for Your Research Learn About ...

...Resources on the Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn...School Students Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check... a multi-gene pathway to study and a reference organism where deletion strains are available. Then, staff...clone similar sequences from a less-well studied organism into overexpression plasmids. Students next transform...transform deletion strains from the reference organisms with the overexpression plasmids. Finally, students...Saccharomyces. cerevisiae, makes a great reference organism for the project because it is non-pathogenic, ...these plasmids, which have been deposited with Addgene, to successfully analyze Met protein complementation...

...in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology...specifically manipulate a cell type of interest in an organism, all cells of that type are affected. Previous...recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount of labeling...versatile and can be applied across cell types and organisms by replacing the transcription factor binding ...the PhiC31 recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable...s41592-018-0184-y Additional resources on the Addgene blog Learn about Gateway cloning Read about genome...

...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...Twitter at @Steph_Guerra Additional resource on the Addgene blog: Browse our blog posts on science career ...

...for all ages. Organize your data and prepare publications or your dissertation Organize data that you...began working remotely recently? Most of us at Addgene began working from home last week to flatten the...new. We usually have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga ...before bed will help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace...virtually connected with the people in your life. At Addgene, we’ve started virtual activities like team lunches...societies hosting virtual networking events. Download Addgene’s Science Career guide to learn more about career...begin working on these drafts and for a few model organisms, Micropublication will accept negative data or...

...  Check Out How Addgene Thinks About Developing Lab Management Software    Organizing and sharing your...share your lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...protocols. Since we began sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning... mobile) tools are available for scientists to organize and share their scientific protocols. Here are...plasmids and you decide to share them using Addgene’s repository, you can also share your associated protocols...gRNA_Cloning Vector (deposited by George Church’s lab). Addgene has also cataloged a variety of useful molecular...

... at Addgene.org Order Plasmids from Addgene! Find Free and Open Educational Resources at Addgene! ...official by registering BosLab inc. as a nonprofit organization in the state of Massachusetts. Because we’re...local companies and BosLab has grown into an organization consisting of over twenty members and volunteers...microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast transformations...Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab, a community biotech laboratory in Somerville... @ZebraElement     Additional Resources on the Addgene Blog Experimenting in an Open Source Lab: from...

...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin... plasmids that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin can be added to...PubMed PMID: 8245422. Additional resources on the Addgene blog In vivo biotinylation of bacterial fusion...

... time to read them. Organizations: Accounts for nonprofits, research organizations, and universities often...hashtags to library entries, which can help you organize and search for papers later. Pro tip Learn more...search terms will show up in your feed. Feeds are organized by papers published each day, week, or month. ...scientific literature. Additional resources on the Addgene blog Learn why scientists should be on Twitter...

... use.  View the Addgene Plasmid Request Data Dashboard! Figure 1: The Addgene Requests Dashboard...I was excited to see that Addgene has recently added a new feature for depositing labs to its website... data on the date, material, requesting PI & organization, and country. This information can be of personal...default. Depositors can download their CSV file from Addgene by following these instructions, and then uploading... 2: Insert showing the upload function for the Addgene Requests Dashboard After the upload, the name...displays the data in a standardized way. Since Addgene encourages the public sharing of aggregated data...data only, information on requesting PI(s) & organization(s) is not shown on the dashboard. Key features...

...your organization Public Speaking Time management Managing a team Conflict resolution Organization Leadership... as Customer Support and Operations Manager at Addgene. In this role, I balance my daily tasks, meet cross-team...than you thought! When change happens at your organization, when your boss needs someone to step up, when...students, managing inventories in the lab, or organizing a research project, one can assume that you’ve...former Customer Support and Operations Manager at Addgene and is interested in nonprofit management and international...development.       Additional Resources on the Addgene Blog Get tips on writing a good cover letter Resume...likely collected some great time management and organizational skills. If you are responsible for working ...

...Awareness Organization (ADAO) are working toward such a ban, and the president of the organization, Linda...Additional Resources on the Addgene Blog Find out More About Cancer and Addgene's Cancer Collection Pages... has spread from where it originated to another organ in the body. Such misdiagnosis and late discovery...tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the surgeon ... Screening in Ghana Additional Resources on Addgene.org Check Out Our Cancer Ressearch Collection Find...

...Nonetheless, the initial release caused a storm as the organization left open the question of how much data was ...to make the underlying data easily available, organized in a form that can be understood by others, balanced...each individual video. To PLOS’s credit, the organization subsequently clarified what the policy intent...researchers. Yet accurate and comprehensible organization of data is essential for efficient science. ...as SlideShare, Figshare and Dataverse. Just as Addgene helps with DNA reagents, these resources can offload...David Crotty (The Scholarly Kitchen) Read more Addgene blog posts about scientific sharing ...

... including the teams at Addgene, ComSciCon, and many more great organizations. At ComSciCon, our primary...your foot in the door Resources on Addgene.org Check out how Addgene uses videos for science communication...respective fields. To help attendees launch new organizations that will extend opportunities to practice science... create them.  Seek out collaborations with organizations that practice science communication or start...entertainment industry.       Additional resources on the Addgene Blog Learn about careers in science communication...

...Protocols Check Out How Addgene Develops Lab Management Software Resources on Addgene.org Find Protoctols ... and do not necessarily represent the views of Addgene. I think it’s safe to assume that anyone reading...me with my research in terms of preparedness, organization, and sharing data. Here are five FREE science...Archives will help both you and your lab stay organized allowing you to upload anything from protocols...came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics...100percentSDS.     Additional Resources on the Addgene Blog Learn How to Keep a Lab Notebook for Bioinformatic...

...engineer a specific function in these multicellular organisms. However, the rewards are great if you take the...are needed to increase the body of knowledge. Organizations like Open Plant can then build on this research...plant circadian clock. Petunias have been a model organism for plant research for decades, and the biochemical...limited distribution. We hope that will change with Addgene’s expansion into plant synthetic biology. There ...tool for plant engineering, please share it with Addgene to both build the plant synthetic biology community...on Twitter Like RevBio on Facebook Read more Addgene blog posts about synthetic biology ...

...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from...that Addgenies represent. They kicked off their day with breakfast and an introduction to Addgene with...to start networking! Learning about careers at Addgene Afterwards, the students split into two groups ...groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology...with the “comfortable surroundings” of the new Addgene office space.  Thanks Abigail, we think it’s pretty...pretty great, too! Thanks to MassBioEd for organizing such a great day, we look forward to hosting more...more students next year! Does your lab or organization do work to help improve K-12 education? Let us ...

...opportunity. That's how Addgene was founded. That's how my position and my team at Addgene was created.”- Eric...discussed on the Addgene blog, consider writing a guest post. Additional resources on the Addgene blog Read...Resources on Addgene.org Find job listings at Addgene Get more career insight from Addgenies ...downloadable guide: the Addgene Science Career Guide. We also asked Addgenies what their favorite career...planning meetings, and finding the best way to organize your time. At a conference, you could be honing...  We touch upon these topics frequently in the Addgene blog. In fact, we have so many blog posts about...find yourself asking, "Why doesn't this product/organization/position exist yet?", you are in the early stages...

...multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads and insufficient...processing mechanism, which is conserved in all living organisms and has previously been used for multiplex Cas9...nuclease editing, in virtually any cell type or organisms of interest with only minimal genetic payloads...detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716...References and Resources More resources on the Addgene blog CRISPR 101: Multiplex expression of gRNAs ...

...the more detailed questions the organizers wanted to touch on in organizer David Riglar’s blog post. Essentially... that there are many companies and organizations (including Addgene), that need people who are data oriented...Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...fun and surprisingly exhausting experience. As Addgene believes many of the topics discussed during the...Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to...skills beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of ...problems within the research environment itself. At Addgene we hope that all parties involved in making academic...