...NGAG and NGCG and SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting domain. However, these alternative...mutants with PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation...recognizing multiple PAMs should have a fitness advantage over proteins with single PAM recognition. After...bacteria where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM...cell population measured with NGS) are shown as percentages, with fold changes compared to SpCas9 in parentheses...

...Wyatt and Dale Ramsden, UNC at Chapel Hill. One advantage to using the CRISPR/Cas system for genome engineering...Persistence of a Chromosome Break Diminishes Its Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org...End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128...

...that controls repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the... 2: The components of Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic modifiers...

...HEKs. These systems built around HEKs offer an advantage to use the cell line and we will highlight some...cell lines are susceptible to contamination by mycoplasma and bacteria, but HEKs are also particularly ...

...the human gut bacteria B. thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. However, many non-model...for CRISPRi transfer Mobile-CRISPRi offers two advantages over existing bacterial CRISPRi methods. First...

...majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves...and prepare fresh media with antibiotics at each stage. The barcodes and LIMS help us track each sample...

...Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed ...Sadowski. 1985. “The FLP Recombinase of the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently...

... chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination...Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae. Paques F and Haber JE. Microbiology...

...GAL4 gene downstream of a tissue- or developmental stage-specific promoter and design a UAS reporter construct...genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197...