... scenarios below for a CRISPR experiment which involves making specific knock-out edits in a gene(s) of... off-target frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes...those sites, especially when DNA mismatches are involved. Two other approaches have been used - GUIDE-Seq...off-targets for non-cutters If your Cas experiment involves activation/repression or any other effect which...

...reading frame is inverted in relation to the promoter, hence the name, double-floxed inverted open-reading...transgene is no longer able to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While...opposite orientation (direction of arrows) the inverted transgene is reverted into the sense orientation...

...postdoc” is, and that “PI” stood for principal investigator. In basic science (as opposed to translational...in a lab. Position Common tasks Principle investigator (PI) Runs the lab Writes a lot of grants Varying...prep media, etc. "Research assistants" are more involved in the science, experimental design, and execution...genuinely excites them, and reach out to the PI to get involved. It not only demonstrates initiative and drive...

...testing and modifications. The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This... the discovery of meganucleases. The storyline involves the resurrection of dinosaurs by extracting the...Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing an anti-villain geneticist named...which goes horribly wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting...

...for). For example, if you study neural pathways involved in addiction (such as the lab I work in), an obvious...constructs have been engineered with a double-floxed inverse open-reading frame (DIO). Upon coexpression with...mouse line or viral co-injection), the opsin is inverted into the correct direction and expressed (under...References 1. Tye KM, Deisseroth K. Optogenetic investigation of neural circuits underlying brain disease ...

... complex DNA constructs, particular for those involving recombination elements such as FRT or Lox sites...of sequences in bacteria are time-consuming and involve many steps. The Drew Endy and Keith Shearwin labs...molecules, allowing labs to precisely and non-invasively control neuronal signaling. For more information...recognition sites that flank a DNA module in an inverse orientation, so that multiple DNA components can...uses two plasmids in tandem, with Cre-dependent inversion determining between 0 and 2 FPs expressed from...

...greater scientific field, potential customers, investors, or other relevant entities or individuals. A ...one that you can pay for, but one that you are invited to join (selected due to expertise in field). Publicity...it as documentary evidence for your case – the invitation to be a peer reviewer needs to be addressed to...diagnostics, healthcare IT, boutique consulting, investment banking and venture capital areas.  We place ...

...I pay the invoice? After your Addgene order ships from our facility, we will send an invoice to the billing... address that you listed during checkout. This invoice will reference your Addgene order number and PO...

... in cancer Previously available methods for investigating and functionally validating the thousands of...useful for? They could be used to more thoroughly investigate any of the dozens of wild-type genes and their...help scientists understand how a given gene is involved in a complex pathway. Some of the most heavily-studied...

...virologists who don't have the resources to get directly involved with COVID-19 to researchers in other disciplines...resources this way, the CCRP2 is giving Rutgers investigators the agility to rapidly initiate complex research... As researchers around the world continue to investigate COVID-19, and once universities reopen and restart...

... S et al., 2002) and many downstream complexes involved, it can be difficult for scientists to understand... be activated downstream of GPCRs that are not involved in sight (Olsen RHJ et al., 2020). A transducer.... Lastly, the activity of both antagonists and inverse-agonists could be measured using the TRUPATH system...

... dissection video. I did that in January. This involves going in front of a camera and kind of walking...operations departments at eScience Labs. This can involve traveling with the sales team to meet faculty and... of determining if projects are feasible. This involves thinking about kit cost and safety.  Being a manager...

...for in vivo behavioral studies because it is non-invasive and offers a high degree of control over time ...larvae of zebrafish are translucent and allow for noninvasive live imaging with fluorescent tags and activation...Wyart et al., 2009). The CPG is a neural circuit involved in locomotion that generates periodic motor commands...

...fairground rides and other fun activities and even invite members of the public to attend. We thought we’...level academic debates for its members to become involved with. The society provides talks and career support... Advice from Addgene Bloggers Learn How to Get Involved in a Profession Organization ...

...key parts of an AAV plasmid. Inverted terminal repeat (ITR) sequence Inverted terminal repeats (ITRs) are...Alexander IE, Logan GJ (2019) A User’s Guide to the Inverted Terminal Repeats of Adeno-Associated Virus. Human...

...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...few months. You can also browse our entire AAV inventory. Our new AAVs include: Optogenetics AAV Control...in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype...

...appears to play a role in many cell processes involving cell membrane dynamics. When they learned there...have some theories on the underlying mechanism, involving the primary cilia. They’ll put a variety of plasmids...gene-edit mutations into XP genes and other genes involved in photosensitivity in melanocytes with the goal...

...  We started with the idea of a biotechnology investment fund that would apply a percentage of returns... is more important than the product itself -- investors would rather fund excellent people with mediocre...Harvard/MIT faculty, venture capital and angel investors, and business development heads at major companies...

...relationship with authenticity and not discomfort: Invite the new contact to connect on LinkedIn and always...make the relationship stronger.  If appropriate, invite them to speak for your organization, mentoring ... simple question so I accepted the connection invitation. They now message me frequently and ask me how...

...impacted by a project. Instead, invite the experts on the topic at hand. Invite one “ambassador” from each ...clarification - new and useful information, and invigoration - a renewed energy for continuing the project...