Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
Type
Blog Post
Published
Nov. 1, 2018, 12:35 p.m.
... in plasmid cloning
Perhaps the most widely used application of CcdB is GatewayTM cloning, where the ccdB...counterselectable marker in the destination vector. Successful cloning occurs when the ccdB gene is replaced by the gene...plasmid, they were able to create an efficient cloning tool. The plasmid pKSCTMF contains the mazF gene...ve also helped researchers design and optimize cloning methodologies using counterselection. Since their...Learn more about CcdB, the toxic key to efficient cloning
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