...more efficiently amplified than larger constructs, but plasmids as large as ~6 kb can be amplified fairly... This is particularly relevant in cases where a large plasmid with multiple functional regions is used... if you want to introduce a point mutation in a large construct. Particularly, if a pair of unique restriction...visible in a digest of the unmodified plasmid into a larger band (not shown). As a footnote; a similar approach...site, can identify these duplications (slightly larger bands sizes relative to the original template)....

...sessions, not your walks between sessions Like many large conferences with multiple sessions happening at ...walking up to the mic and asking your question in a large conference room puts butterflies in your stomach...

...recommendations from the depositing lab. If your vector is large (>10 kb) or has a low copy number, try following...agar plates, you may notice that there are both large and small colonies. Bacteria containing the recombined...

...image nascent proteins. In addition GFP tags are large, dim, and permanently attached to a protein, making...else can you do with the HA frankenbody? Another large advantage of the HA frankenbody is that it remains...

...story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start, the average...different than what was originally planned, due in large part to the feedback we received from our network...

...optimized by testing various linker configurations in a large-scale functional screen in bacteria. Sensor variants...quite a robust way to make a FRET sensor with a large dynamic range,” he says. Merkx recently applied ...

...and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in optimizing...required per system. Applications of these systems The large dynamic range of these systems ensures that they...

...thing to consider is the size of the conference. Large conferences have many opportunities but can be overwhelming... overwhelming as far as meeting new people. At large conferences, look for smaller events or meet-ups ...national recognition and the more people you know, the larger your profile in the community Job hunting outside...

...Musunuru: We have been using the kit to target a large number of genes involved in cardiovascular and metabolic...power here is that you could potentially sequence a large number of individuals and find several mutations...

...sequence, or if you want to amplify sequences from a large genome organisms like mouse or human. There are ...difficulties in amplifying a template, particularly from a large genome, you can recheck your primer design or optimize...

...time intensive to process a large number of microbiome samples. To enable large-scale, longitudinal studies...

... scientists need a protease reporter that has a large dynamic range and high brightness. The Shu Lab from...FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter...

...site in the genome you are trying to target play a large role in Cas9 activity and efficiency (Sander and... structure and genomic recognition sites play a large role in CRISPR-Cas9 efficiency. They continued to...

... repositories like Addgene provides large-scale savings. How large? Using Doench’s calculations and the...

..., gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases...frequency of both indels at each locus, as well as large deletions. They detected fragment deletion with ...

... to choose from that sequence preferences must largely be ignored. Created with BioRender.com. ...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using...Tsherniak A, Iorio F (2019) Agreement between two large pan-cancer CRISPR-Cas9 gene dependency data sets...

...new way through the integration of science with a large set of other societal pressures including economic...p” policy is non-governmental work happening at large organizations who have to develop policies for their...policy to combat sexual harassment in academia or a large company who decides how they are going pursue regulatory...what better place to do that than at the nation’s largest integrated healthcare system? Defining your why...

...previously-reported technique for precisely integrating large DNA sequences into a genome (Anzalone et al., 2022...deletion, replacement, integration and inversion of large DNA sequences with twin prime editing. Nature Biotechnology... (2024). Efficient site-specific integration of large genes in mammalian cells via continuously evolved...

...input to an RSM drives expression of a distinct large serine recombinase that recognizes its own attB ...high copy, ColE1 origin of replication), contains large serine recombinase genes downstream of inducible...faster or more efficiently than others. Multiple large serine recombinases have been described in the literature...