...centrifugation (lenti) Centrifugation Titration time ~1 day (AAV); ~1 week (lenti) 2–10 days Initial titers Higher...will help you decide which is right for you. Table 1: Mammalian versus insect cells for viral production...the insectspodoptera frugiperda (lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007...

...environments such as live cells and tissues (Figure 1). These flexible strategies give you the best of both...majority of in-cell labeling strategies have two parts: (1) a genetically encoded “tag” expressed as a fusion...Follow him on twitter @rhodamine110.   References 1. Liu, Zhe, Luke D. Lavis, and Eric Betzig. "Imaging... tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be ...

... are not required to initiate the reaction. Table 1 compares some general advantages and disadvantages...disadvantages of luminescence versus fluorescence.  Table 1: Properties of Luminescence versus Fluorescence   ...None Yes Luminous Japanese ostracod(Cypridina noctiluca)Sea firefly(Cypridina hilgendorfii) 465 62 Cypridina...

...prompted us to create Crowdfight.   Figure 1: Crowdfight is a community of scientists who believe...collaborator for a specific task, Crowdfight will: 1) Find an expert with the right skill; 2) Confirm the...IgnacioAmigoH     María Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica...

...animal will repeatedly nose-poke at the active hole [1, 2].    Place preference/place aversion also models... can be explained primarily in two different ways 1) your hypothesis is wrong or 2) there is some problem...follow him on Twitter @derekpsimonphd.    References 1. Stuber, Garret D., et al. "Excitatory transmission...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

...occur, but good performers move around easily  Table 1: Taking it a Step Further - Big Company v. Small Company...recently, I have been hearing exit survey data from postdoctoral programs in the Boston area that demonstrate...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless...

...total dose between 1 x 1011 and 5 x 1011 vg per animal (i.e., 10 and 50 µL of vector at 1 x 1013 vg/mL titer... and 1 x 1012 vg*. Most systemic capsids are easy to produce in high titer, but higher doses (>1 x 1012...before using it in an application.     Figure 1: AAV toolkit for targeted gene delivery to the brain...decision tree for selecting a capsid, while Table 1 gives a brief overview of published descriptions of...Capsids page to learn more about that collection. Table 1: Systemic capsid plasmids available from Addgene ...AAV9. At lower viral titers than AAV-PHP.B (e.g., 1 x 1011 vg/mouse), AAV-PHP.eB can transduce a greater...Diverse Brain Cells. Annual Review of Neuroscience, 45(1), 447–469. https://doi.org/10.1146/annurev-neuro-111020...

...detects samples at clinically relevant concentrations (1-3 fM; 1x106-fold lower than before), and our combined... health, energy, and sustainability.   References 1. Pardee, Keith, et al. "Rapid, Low-Cost Detection ... switches for pathogen detection In 2014, as postdoctoral fellows with Jim Collins and Peng Yin at the...

... to the development of resistance include (Figure 1): Over-prescription of antibiotics (for example, ... communication, and public health.     References 1. O'Neill, J. "Tackling drug-resistant infections globally...www.youtube.com/watch?v=sx1uDYSfINA. When you go to the doctor, it will very likely take time to determine if ...

...cereblon, leading to rapid target degradation (Figure 1). Plasmids: We generated a series of plasmids that...contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted...studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber Cancer Institute. ...

...single neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP) and...genOway press release for more information. References 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M. Sternson...guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question...

...commandments for making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are ...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...

...education, government, and civilization in general (1). Science and politics? Science is an apolitical process...consequences of a march. Three of these risks include: 1) Lost trust Perhaps the greatest risk is that the ...active research scientists (scientists who hold a doctorate degree, are currently working full-time, and have...

... such a popular model organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate ... post was contributed by Katherine Rogers, a postdoctoral researcher at the Friedrich Miescher Lab of ...Max Planck Society. Katherine W. Rogers is a postdoctoral researcher in Patrick Müller’s group at the ...

...blood test for HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the... for years, or they are exposed even one time. Doctors and researchers don’t entirely understand why some...exposed to asbestos Tests like these will allow doctors not only to detect mesothelioma much earlier, thus...

...could be kept frozen after collection. Figure 1: Paul included a voltmeter on top of the battery box...Thermus aquaticus bacterium – was discovered at Octopus Hot Spring at YNP, and since then, many researchers...

...breaks (DSBs) or donor DNA templates.  Figure 1: An overview of prime editing. Created with BioRender.com...demonstrated ~20-50% efficiency in HEK293T cells with 1-10% indels. Since then, further innovations have continued...Engineering. https://doi.org/10.1038/s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward...post was originally written by Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December...

...SpCas9 base editors with editing windows as small as 1-2 nucleotides! To reduce off-target effects associated...Lower on-target activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very ... through the expression of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model...Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription activation ...

... After all I was only going to be interviewed for 1 hour so having a broad enough insight was going to.... I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge and knew...

...process is explained more fully in the animated Fig. 1 below. Normalization is a fundamental and often understated...Bioinformatics from Boston University, and did postdoctoral work at Harvard Medical School and the University...