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Showing: 641 - 660 of 802 results

  1. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ... can clone the genes from the hybridoma into a plasmid to create a recombinant antibody.     Figure...Resource on Addgene.org Check out Addgene’s antibody plasmid collection Read the latest on Addgene's new antibody...

  2. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...open doors to a web of opportunities that the classroom cannot offer. Furthermore, companies across most... letter that is customized for each internship. Lastly, have your resume and cover letter revised by your...

  3. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...be from the same host species, have the same Ig subclass, and be in the same fluorophore (e.g., if you ...staining for anti-mouse Ly6C in APC with the IgG2c subclass, you will use the APC IgG2c isotype control). ...

  6. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...for an undergrad taking their first upper-level class. Even so, it’s quite easy (and common!) to be blind... can understand and process your information. A class of three year olds is unlikely to be able to follow...

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...recombinant plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection...

  9. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...contain two numerical designations, separated by a slash, that indicate the ITR and serotype, respectively...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....

  10. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ... the lab. What is a Fab Fragment? Consider the classic IgG antibody (Figure 1): it is composed of four... (more on when that might be later). Find scFv plasmids at Addgene Depending on how an antibody is broken...

  11. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ...RNA molecules are vulnerable to attack by both cytoplasmic and nuclear nucleases, as they will be recognized...ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources on the Addgene Blog  How...

  13. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ... create fusions with a gene of interest. These plasmids will be most useful for constructing intermolecular...PMID: 27475975. 6. Gayrard, Charlène, and Borghi, Nicolas. "FRET-based molecular tension microscopy." Methods...

  14. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...course, UWE Bristol offers an intensive 4-day Masterclass in science communication. The National Co-Ordinating...materials and assignments.  9) Citizen science projects Lastly, if you'd like to directly involve the public in...

  15. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...catchy, but beware. A distracting background or clashing colors can take away from what should be the focus...single-color background but want to add a bit more “flash,” you can include a subtle gradient (as is shown...

  16. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ... Addgene’s experience helping scientists share plasmids, especially those of the CRISPR variety (see slides...is in one of the top 10 countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic...

  17. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise changes in histone...Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors...

  18. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...methods for the determination of amplification of Plasmodium falciparum multidrug resistance-1 gene (pfmdr1... RJ, Paterson ES, Smith ML. The copy-number of plasmids and other genetic elements can be determined by...

  20. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... The most obvious workaround is to use a multi-plasmid system, where you have one AAV vector dedicated...interrogate immune cells to improve immunotherapy in glioblastoma, and generate programmable screening platforms...
Showing: 641 - 660 of 802 results