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  2. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...and Paul Greengard. "Dopamine-sensitive adenyl cyclase: possible role in synaptic transmission." Science... Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate nucleus of rat brain, and its similarity...

  4. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...size, structure, and other qualities of each tag class, they are generally best suited for their primary...Antibody protocols  Resources on the Addgene blog Plasmids 101: Protein tags Antibodies 101: Epitope tags...

  5. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ... 12–15 kDa Ease of cloning and expression from plasmids, due to only needing one open reading frame Ability...Membrane Proteins More resources from addgene.org  Plasmids encoding nanobodies at Addgene Sybody Generation...

  7. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...strain used). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over 100 entries...expression after AAV injection, what region and cell class ended up expressing your virus? If you were using...

  8. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...Nathaniel Roquet from the Lu Lab at MIT developed plasmid based systems that report the order in which specific... plasmid...

  9. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...accelerate your research.   Why distribute virus? Plasmid distribution is booming, but, over the years, many...list of what’s available or suggest additional plasmids with the Viral Service Suggestion Form. Lentivirus...

  10. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ... through a structured environment such as the classroom or a defined learning program. Learners cannot...high school who are also students in AP biology classes Creating a podcast about genetics using dog ...

  11. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...course involved a whirlwind of skill acquisition. Classes covered filmmaking, podcasting, philosophy of science... Ramanujan, to be precise) and remarking to a classmate that, if this was one day required of me for work...

  12. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...Extracurricular Activities Professional Skills Open source classes (e.g. edX, MIT OpenCourseWare) Access to knowledge... (e.g. Volunteer at a local high school science class: Science Club for Girls) Communication to diverse...

  13. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching space at Lab Central to learn...drawstring backpack and a double wall insulated glass to take home with us. Tips to to get the most out...

  14. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...the MG-2I-dL5** FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation...Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser inactivation in cell biology. Trends in Cell Biology...

  15. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...poetry, and I’ve been reading a poem a day for the last 8 years. Since then, we’ve read books across nearly..., true crime, and so on. We’ve read well-known classics, like Austen’s Pride and Prejudice, and some more...

  16. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ... are many things we can navel-gaze over from the last couple of weeks, including whether or not scientists...Addgene.org Read our CRISPR guide Find CRISPR plasmids by function Check out our genome engineering guide...

  17. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...tRNA-gRNA arrays, which is scalable and efficient in plasmid and viral vectors. In applications when repetitive...manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716).   ...

  18. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ... between two halves of a bacterially derived periplasmic glutamate binding protein known as GltI. When...virus aliquots being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene...

  19. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...validated editing reagents can be found on our plasmid pages. We encourage any labs performing genome ...CRISPR Background with Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our List of gRNA Design...

  20. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...spectra of your protein with the filter sets or lasers on your microscope. Ideally, you would like to ...Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 / 640...
Showing: 621 - 640 of 801 results