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Showing: 581 - 600 of 757 results

  1. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ... Figure 1: SARS-CoV-2 proteins and other components. Image from Maya Kostman for the IGI.     ...-LAMP) and a colorimetric readout that can be completed in just 45 minutes. The team deposited the reverse...

  2. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...expanding our ability to study and understand the complex interworking of the 86 billion neurons in the human...Sciences. Like many split FPs, split-wrmScarlet is composed of two FP fragments that are not fluorescent on...

  3. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    .... The donor and acceptor fluorophores must be compatible, such that the emission spectrum of the donor...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...

  4. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... for us all.  For example, implementation and compliance for the FLSA changes, including finding money...postdocs nationwide. On this level, FoR is currently compiling a database of institutional responses to the FLSA...

  5. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...few new ones that are not used so much but have comparable expression levels to things people do use a lot...example, knock out a gene with simultaneous complementation, with a knockout and expression construct on...

  6. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...practice while seated or with your poster on your computer screen than it is to pretend you’re standing at...guilty of this, and have many times answered a completely different question than the intended one. Take...

  8. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...or producer cells for further processing. What component(s) you need to collect will depend on the serotype...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

  9. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...protein fusion. CRISPR genome editing can help accomplish this goal. The power of this system lies in the...PMID: 27052166. Kunzelmann, Stefan, et al. “A Comprehensive Toolbox for Genome Editing in Cultured Drosophila...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s...particular cell type, or a subset based on a cellular compartment or growth stage). A prey library can either be...

  11. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...literature and emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal...gauged interest from relevant parties, and the company at large, by polling and informal communications...

  12. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...

  13. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology...time. Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of...

  15. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ... or very similar buffers if not, since buffer components can affect the final readout. When preparing ...using advanced graphing software designed to plot complex trendlines. If you don’t have access to this kind...

  16. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out...

  17. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...roughly measures granularity, a proxy for nucleic complexity of the cell.  The sample will have been labeled...labeled using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/...

  18. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

  20. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...varied codon preferences among organisms aren’t completely understood, but some possible reasons include...codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online codon optimization...
Showing: 581 - 600 of 757 results