...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection...collection Read our molecular biology reference Find plasmids for your research ...

...profile problems surrounding cell culture over the last few decades some pharmacologists have tended to ... information in your lab book and on the actual flasks of cells in the incubator). This means any other...Protein Localization Resources on Addgene.org Find Plasmids for Mammalian Cells ...

...varying between 10-50°C. Visit Addgene's COVID-19 Plasmids and Resources Many thanks to our guest bloggers...more about SHERLOCK and DETECTR Learn more about plasmids available for COVID-19 research Resources on ...in our CRISPR guide Browse our entire COVID-19 plasmid collection ...

...-time, and have received a research grant in the last five years as polled in the American Association... funding for science has been decreasing for the last five years and the first federal budget request ...March for Science Website Locke, John, and Peter Laslett. Locke: Two treatises of government student edition...

...You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target...from a variety of vendors and you may even find plasmids encoding antibodies from Addgene that you can ...cellular compartments such as the mitochondria, cytoplasm and nucleus.  We hope that this blog has provided...

...experiments? In this post, we will discuss some broad classes of optogenetic tools, and what factors to consider...using optogenetic tools Once you’ve selected the class of tool appropriate for your experiment, you’ll ... https://doi.org/10.1038/nmeth.1808 Nagel, G., Szellas, T., Huhn, W., Kateriya, S., Adeishvili, N., Berthold...

...demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move toward the cell...be easily performed too. While I implied that axoplasmic transport of AAVs is a potential problem in many...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

... models perfer the PA-RFPs over PA-GFP. Another class of irreversible PA-FPs are the photoconvertible ... (level of zoom, intensity, possible excitation lasers) it is also important to optimize your activation...Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting...

...mitochondria, and different dyes can result in varying cytoplasmic background signals when used with the same protein...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...More resources from Addgene Fluorescent protein plasmids and resourcesA practical approach to choosing ...

... a few gRNAs suggested to be effective by world class CRISPR labs.  Check Out This Video to See How Green...particular, one slide of the presentation made a lasting impression on me. The slide showed a complex signaling...Find CRISPR Screening Libraries Browse All CRISPR Plasmids ...

... making fiber optic cables only to hook up your lasers and not have the proper controls or a proper behavioral...surgeries. Identify coordinates through a rodent brain atlas or research papers and practice with die injection...PMC3314244. Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein ...

... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...2021). TGFβ1 induces resistance of human lung myofibroblasts to cell death via down-regulation of TRPA1 ...

...about that collection. Table 1: Systemic capsid plasmids available from Addgene Capsid Published ...re interested in. Then request your capsid (or plasmids) and let the real work begin — validation, validation...2021). Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron,109(9), 1449–1464.e13... P. (2021). Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex...

...received the first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that ...

...Deckthelab contest. The bar was set very high from last year’s impressive entries, but our community of ...2017 #Deckthelab winners. We hope everyone had a blast decorating and creating memories with their labmates...

...trying.  When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and...addgene.org! Fun fact! Did you know you can search for plasmids from a publication by pasting the paper's title...

...topic or lab process. Along with our role as a plasmid repository, Addgene aims to be a vital educational...Addgene.org Check Our Our Protocol Pages Browse our Plasmid Reference Pages ...

...Baker, who was awarded the Nobel Prize in Chemistry last week alongside Demis Hassabis and John Jumper! The...evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with other tools like high resolution...

...yield and quality of the viral preps. For example, last year we published an article describing a sucrose...instructions and calculations on how to prepare a plasmid stock used to generate a standard curve, visit ...

... Data repository From the Human BioMolecular Atlas Program (HuBMAP)  Antibody Characterization ...imaging Data repository See blog post mAb3D Atlas Mouse and human brain  IHC Data repository...