... 96 parts including terminators and promoters, CRISPRi parts, selectable markers, transformation acceptor...promoters, and inhibited gene expression using CRISPRi. The kit functions in the model cyanobacterium ...

...or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed to study the...

...practices could be an actionable way to tackle widespread irreproducibility. Luckily, there were other ...practices could be an actionable way to address the widespread issue of irreproducibility. Use your network...

...our “google doc of gratitude.” We must keep spreading the word about materials sharing, but we don’t... who support us by depositing, requesting and spreading the word. Our blog and site resources are just...

...transient gene expression in a particular cell type to CRISPR genome editing, optogenetics, and chemogenetics...different ages." Retinal Degenerative Diseases. Springer International Publishing, 2016. 501-507. PubMed...

...Split-BioID plasmids Find CRISPR plasmids for labeling genomic regions Find CRISPR plasmids for purifying...

... position the tips indepently making it easy to spread out your samples. This can be useful if you’re ...plates onto agar q-trays where the samples must be spread much farther apart before plating. With this fine...

... observed light emission by fluorescence. The widespread use of FRET with fluorescent molecules, including...cascades, quantify mechanical tension (a molecular “spring”) (Meng et al., 2008), and monitor neuronal activity...

...intracerebroventricular) to minimize tissue damage; however, the spreading of vector to the area of interest may be very ...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency...

...For instance, through open plasmid sharing, the CRISPR community has created hundreds of useful variants...Antibodies: a Laboratory Manual, 2nd Edition. 2104. Cold Spring Harbor Laboratory Publications, New York. Helma...