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We narrowed to 218 results for: sms

Showing: 41 - 60 of 218 results

  1. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...developing novel technologies to analyze molecular mechanisms of genome functions such as epigenetic regulation...epigenetic regulation. To elucidate molecular mechanisms of regulation of genome functions, we need to...will markedly accelerate research of regulation mechanisms of genome functions in the hope of developing...

  2. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ... a wide range of organisms and cell lines. In C. elegans, one of the model organisms which I use to study...methods – could greatly streamline editing in other organisms and systems, allowing rapid progress in a wide...

  3. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...vectors to study the neural circuitry behind the mechanisms of sleep and anesthesia. In Cre transgenic mice...optogenetics to understand the cellular and molecular mechanisms of the brain in vivo. Many thanks to our guest...rhodopsins: structures, functions, and molecular mechanisms." Chemical reviews 114.1 (2013): 126-163. PubMed...

  4. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...organs, and most importantly escape the immune mechanisms of the host. The cause, manifestation, diagnosis...this phase are able to outsmart the host immune mechanisms altogether and escape future immune surveillance...of colorectal cancer. Strategic immune evasion mechanisms - The tumor microenvironment Through interactions...

  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...plasmids due to lower activation of DNA-silencing mechanisms. While minicircles are typically smaller than...) studies focused on better understanding the mechanisms of reprogramming, and 2) studies with clinical...Hochedlinger. "Induced pluripotency: history, mechanisms, and applications." Genes & Development 24, no...

  6. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ...listed? Help us serve you! Let us know about other organisms we should include by emailing us at [email protected] narrow your keyword search to one of these organisms. Help us continue to develop resources for your...

  8. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...

  9. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ... editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms...obligate thermophiles without the need to grow the organisms at lower than optimal temperatures. GeoCas9’s ...

  10. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...versatile and can be applied across cell types and organisms by replacing the transcription factor binding ...the PhiC31 recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable...

  11. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...cleavage. Secondly, fluorescence imaging of living organisms can be challenging due to issues with tissue autofluorescence...dynamics of apoptosis both in vitro and in living organisms to better study and understand these diseases ...

  12. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional...for the advancement of scientific research, and mechanisms to maintain and grow these critical organizations...

  13. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ... can function to break membranes of different organisms. And finally, we demonstrated that R bodies can...speculatively, R bodies might endow divergent organisms with new functions. For inspiration, we can look...

  14. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...Viral delivery has several advantages over other mechanisms. For one, many viral platforms, such as AAV or...analyte. This can be difficult to achieve in whole organisms, but AAVs make it possible. In AAV systems, different...

  15. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be...

  16. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...basics.  Mesophiles vs. extremophiles Mesophilic organisms are your regular next-door microbes which grow...cold conditions. However, many of the detailed mechanisms that allow extremophiles to survive under these...

  17. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ... Fig. 1 CRISPR activator and repressor mechanisms Want to transiently induce expression? Check...post-translational level, unlike most of the other mechanisms described. Fig. 3 dTag mechanism of protein...

  18. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...

  19. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...Prokaryotic termination   Prokaryotic termination mechanisms fall under two general categories: rho-dependent...dependent, Polymerases I and II employ different mechanisms to terminate transcription. Polymerase I uses...
Showing: 41 - 60 of 218 results