... for reduced off target editing, such as SpCas9-HF1, eSpCas9(1.1), or HypaCas9. Read more about Cas9 options...Tools Read other CRISPR blog posts Find CRISPR Plasmids at Addgene  ...

.... citrea scarless editing plasmids Sheng Yang’s lab describes a two-plasmid system that combines recombineering... lab M. tuberculosis CRISPRi plasmids Noting that the more common spCas9 system has generally low efficiencies...engineering functions in bacteria, but have no fear - plasmids from Addgene depositors are making it easier than... system available from Addgene consists of two plasmids: pCas9: carries Cas9 and chloramphenicol resistance...gRNA and a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" after induction...editing takes two days, and the pTargetF and pCas plasmids can be subsequently cured from the bacteria. Although...but has many other advantages including shorter plasmid curing time. pCRISPomyces for editing in Streptomyces...

...recently that researchers began building optimal plasmids to harness the power of Class 1 systems. Class...Lastly, type IV systems are most often found on plasmids. The many unique features of IV-A and IV-B have...led to the hypotheses that they are involved in plasmid competition or can hijack machinery from other ...There are three subtypes, A–C, with the beloved SpCas9 (from Streptococcus pyogenes) belonging in II-A...highly diverse and involved in competition between plasmids. Nucleic Acids Research, 48(4), 2000–2012. https... resources on Addgene.org CRISPR guide CRISPR plasmids CRISPR references ...

...technique was applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush...

... Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...inducible inactivation. Find the Robinson plasmids or the Royle plasmids and get to work! Robinson et al...designed the pOSIP (one-step integration plasmid) series of plasmids, vectors that convey both the sequence...Yonglong Chen, pCS2-3xFLAG-NLS-SpCas9-NLS is a Cas9 expression plasmid that was used by Chen and colleagues...mGRASP plasmids. Additionally, the lab recently described the use of another set of mGRASP plasmids in their... favorite plasmid technologies? Email us at [email protected]  and let us know what plasmids you would ...sgRNA libraries expand upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA...