...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...conference format. Additional resources on the Addgene blog Read all of our blog posts about conferences...scientific conferences have followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended...conferences (TAGC and ASGCT, respectively) and Addgenie Angela Abitua hosted a virtual Reproducibility...

...Viral Services Resources on the Addgene blog Viral Production at Addgene Viral Vectors 101: What is a Virus...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral...   Is there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...

...reading, and organizing Tips for writing a scientific review article   Write for the Addgene Blog ...also another free citation manager that lets you organize and cite papers. With the Zotero Connector, papers...managers. If you want more tips on finding and organizing papers, check out this related blog post: Early...Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear...method in grad school Additional resources on the Addgene blog: Tips for writing scientific manuscripts:...

...many organisms. When supplemented with adenine analogs such as 2-fluoroadenine (2-FA), organisms with ...Zurich. He is fascinated by how organisms exquisitely and robustly self-organize at the cellular and multicellular...Resources on Addgene.org Find tools for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids...Fortunately, researchers studying plants and other organisms lacking this innate immunity need not worry and...work is required, several vectors can be found at Addgene that could serve as an excellent start (#22988,...many tools for shRNA in mammals are available at Addgene. Identifying actively silenced plants Once you’...you’ve introduced your RNAi into your organism, how do you identify the actively silenced ones? Here, I’...

...has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double stranded...diseases have connections to either specific organisms or organism/gene interactions with the host. This scenario... in bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use...CRISPR-induced double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage...CRISPR system in E. coli. The system available from Addgene consists of two plasmids: pCas9: carries Cas9 ...can consider the microbiome as a collection of organisms, their genes, and metabolic processes, occupying...to diseases where the presence of just a single organism, such as Clostridium difficile, could be targeted...

...plasmids with Addgene. These can be used to express genes in a variety of model organisms. Use the links...Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources on the Addgene Blog Try Out... attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose...expression system in just one recombination step. Addgene’s ready-made entry clones can be used with a large...of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Set up your own Science by the Pint series and organize evens where scientists can grab a beer and chat...discuss the importance of actionability on the Addgene blog and how SITN short-form blog posts help readers...institutional knowledge and continuity - two lead organizers on each program, one junior and one senior.  ...For more info about starting your own SciComm organization and to learn about some of SITN's other initiatives...Additional Resources on the Addgene Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect...

...to ensuring plasmid quality is to stay organized. Here at Addgene, we collect data on every plasmid we ... Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View our ...the best things about sharing plasmids through Addgene is that we provide an added level of confidence...can take to verify your own plasmids. Staying organized With hundreds of samples coming through our doors...selection markers. You don’t need a fancy database to organize your own plasmid collection, although you will...physical sample and its associated information are at Addgene, we are ready to sequence verify the plasmids. ...plasmid you are working with, but, in most cases at Addgene, we check what we call the “insert,” (the gene ...

...tool to advance our knowledge of brain-visceral organ signaling. The problem  Previously, there were... for monitoring how both the brain and visceral organs interact during various behaviors. Either tissue...common neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar...applications are to expand the tool to different brain-organ axes. Alternatively, the system could be used to...initiative plasmid collection More resources on the Addgene blog Plasmid tools for microbiome studies Channel....org/10.1038/s41587-023-01833-5 Resources on Addgene.org Optogenetics guide Chemogenetics guide BRAIN...

...application. Even within an organization, there can be variance. At Addgene, our content team, where writing...asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab data so...explain why you are interested in working for that organization or group. This is particularly important for...title of the position] at [name of the company/organization]. I am a [adjective that describes you] scientist...updating this post, I took an informal poll of Addgene hiring managers. All of them stated that a good...that particular job, your commitment to the organization's mission, or your deep interest in the lab's... in May 2024.     Additional Resources on the Addgene Blog Learn to Develop Your Professional Network...

...also our mission here at Addgene! Along with other open-science organizations, we support scientists in...their CV or grant applications. A number of organizations (such as ASAPbio) advocate for preprints, and...manuscript to a journal for formal peer review. Some organizations, including some journals, also support open ...general public (say, policymakers or community organizations) to use your work but don’t think they’ll have...the manuscript prior to submission. Preprints at Addgene Preprints are all about sharing scientific knowledge...significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in the final journal...10.1371/journal.pbio.3000116 More resources on the Addgene blog Summer SciComm Series: Modes of Communication...

...plants available through Addgene. In addition to the plasmids described above, Addgene carries several useful... ease of use and utility in a wide variety of organisms.  While much of current CRISPR research focuses... plant species, including commonly used model organisms like Arabidopsis and Medicago truncatula and several...universal functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components... are available to academic researchers through Addgene. CRISPR components for plant genome engineering...experimental design principles developed in other model organisms (see our CRISPR guide for common CRISPR principles...sequence) is consistent between plants and other organisms, but the promoter used to drive gRNA expression...

...critical in the business world. Companies and organizations that avoid all conflict can fall into “groupthink...individuals involved, and also for the labs, organizations, or businesses as a whole. When I’m attempting... sharing gets more challenging the bigger an organization gets, and creating new avenues of communication...should be communicated is necessary in a large organization, because confirming that every person has read... a common source of conflict. For instance, at Addgene, there are two teams whose primary role is customer...attempting to resolve conflicts within a given organization, it can help tremendously to have as much context... solutions that benefit themselves and their organization. Resolving conflicts is great, but avoiding ...

... available in your lab or organization, and approvals from the organization overseeing your work. Good... on the Addgene blog Mouse Modeling Part 1 Mouse Modeling Part 2 Five Popular Model Organisms Additional...the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular choice for administering...and to avoid potential off-target pokes to nearby organs. Restraint: scruffing Location: peritoneal cavity...Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids...

...your current organization but also important to build relationships outside your organization. The mentor...mentors all around you. This third article in the Addgene Blog Mentoring series will cover 2 of the 7 mentoring...what makes a good mentoring program. If your organization has a mentoring program, consider tapping into...upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking someone...

... the kits Addgene offers to kickstart these applications, no matter what your model organism is. MoClo...across organisms. When using the MK system, many of the parts can be used in a variety of organisms, saving...019-46171-2. Resources on Addgene.org Modular Cloning (MoClo) Guide Addgene’s eBook Collection More resources...MoClo kit recommendations for different modal organisms and applications.    Plant applications We...starter for your general plant cloning experiments. Addgene also has two additional MoClo plant kits: Plant...libraries and tools available for certain model organisms and backgrounds, MoClo can be invaluable for more... complex editing and screening experiments in organisms that don’t yet have these tools built out. While...

...10.1186/s12870-020-02388-2 Resources on Addgene.org Addgene’s Modular Cloning Guide EcoFlex MoClo Kit...modular cloning? Modular cloning, or MoClo, is an organization scheme for multi-part DNA assembly. Simply put...other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical...with characterized behaviors are available from Addgene and other sources; second, characterizing a part...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...MoClo Kit MoClo Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...from Science in the News, a graduate student organization that works to promote science communication....non-scientists. These programs are all run and organized by graduate student researchers. That’s right,...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...

...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from.   Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau, R.W., Lee-Gosselin, A....PubMed PMID: 29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...