... Ten simple rules for collaboratively writing a multi-authored paper. PLoS Comput Biol 14:e1006508 . https...

...page simplifies the process of building advanced multi-parametric search queries.  Looking for a bright...Protein Choosing Your Fluorescent Proteins for Multicolor Imaging Resources on Addgene.org Browse all...

...approach as a tool for monitoring multi-gene expression and adapt it for multi-color calcium sensing (plasmids...Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. Multi-bit genetic memory One of the goals of synthetic... bits in order to achieve accurate and reliable multi-bit memory. To this end, Chris Voigt and colleagues...distinct emission spectra suitable for single cell, multi-color imaging. The Nano-lanterns rely on the principle...toolkit based on the Multisite Gateway® technology from Life Technologies. The MultiSite Gateway Kit is ...recently developed a CRISPR-based multicolor tool enabling the imaging of multiple endogenous genomic loci simultaneously... innovative ways. The principle of protein multimerization is seen in many important biological contexts...

...Generating constructs with multiple point mutations NEBuilder HiFi can simplify multi-position site-directed...expediently join multiple synthetic fragments, create multiple mutations in one or multiple fragments, and...joining multiple fragments of DNA to create a synthetically designed DNA sequence. There are multiple methods...Assembly Mix include:  Generation of constructs with multiple point mutations Cloning sgRNAs Production of Yeast...site-directed mutagenesis by assembling multiple fragments containing the desired mutations. This same process can...with linearized vector for seamless joining of multiple mutations spread over thousands of base pairs....CRISPR experiments can be laborious, requiring multiple cloning steps. You can simplify this process by...

...researchers are preparing and carrying out this multi-pronged attack. Inflammation and tumorigenesis ...

...assembly for turning the single-input reporters into multi-input cell classifiers in one assembly step. Find...

...specific ratio of products is needed, such as within a multi-protein complex, stabilized promoters can eliminate...fluctuations. When cells are dividing rapidly, multiple rounds of replication are initiated before the...

...forward genetic screen using CRISPR libraries is a multi-step process (see Fig. 2). In most cases, CRISPR...any experiment, the use of appropriate controls, multiple replicates and several cell types can strengthen...strengthen your results. Enrichment or depletion of multiple gRNAs targeting the same gene can be strong evidence...

...mechanisms by which proteins assemble into functional multi-subunit macromolecular complexes, but a serendipitous...concentrations (≤3 mM) of GdnHCl can functionally rescue multiple Arg-mutant proteins in living baker’s yeast cells...

.... A high affinity terminator may be desired for multi-cistronic constructs where high termination efficiency...Polyadenylation is the post-transcriptional additional of multiple adenine (A) nucleotides to the tail of a messenger...PMC104046. Wodrich H, Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey virus constitutive...

...that bacteria usually cannot produce functional multi-domain mammalian proteins as bacterial cells are... expression of mammalian proteins that require multiple post-translation modifications for proper protein...

...RNAi experiments. Notably, this plasmid harbors a multi-cloning site flanked by T7 promoters to allow for...

... is straightforward. Instructors first select a multi-gene pathway to study and a reference organism where...occurs through a well-characterized pathway with multiple enzymatic steps (5). In their experiments, students...Find the Plasmids Here Plasmid features enable multiple student experiments We used TOPO cloning to insert...

...conformation. Because many aaRSs exist within a multi-tRNA synthetase complex, the team investigated whether...SARS-CoV-2 by mutational scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the...which required protein compartments to co-localize multiple enzymes in a reaction pathway, the MPyV system...

...applications of CRISPR including: generating single or multi-gene knockouts in a wide variety of cell types, ...

...single-component nuclease to mediate cleavage rather than the multi-subunit complex employed by class 1 systems. A putative...vectors. In 2017, Zetsche et al. developed a Cpf1 multiplexing approach using a single crRNA array to express...varies widely with gRNA sequence. Both groups used multiple methods to examine Cpf1 off-target editing. First...publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing by CRISPR–Cpf1 Using a Single crRNA...

...adjusting the thickness of the laser sheet and using multi-view imaging can provide enhanced resolution (8,...combined light sheet microscopy with sequential multiple-view reconstruction (5). In order to image large...light scattering and absorption.  Finally, the photomultipliers used to detect light in confocal microscopes...acquisition is performed using cameras with electron multiplying couple-charged device (EMCCD) or complementary...through Addgene.  The combination of LSFM with multiple tissue clearing strategies and neuronal labeling...

...Barbara, et al. "Gene optimization mechanisms: A multi‐gene study reveals a high success rate of full‐length...codons unaccounted for. The result is redundancy; multiple codons encode single amino acids. Evolutionary...

...cells expressing both dCas9 and gRNAs. The multi-gRNA piggyBac vector integrates into genomic DNA...https://doi.org/10.1016/j.pep.2020.105607 A new multiplexed CRISPRi and CRISPRa system for human pluripotent...uniform and sustained expression of single or multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator... transcript and protein levels. Plasmids for multiplexed piggyBac gRNA delivery and the all-in-one dCas9...

...Filtering for Off-target Effects We first generated a multi-fasta file of the hg19 genome using Bedtools getFasta...