...regulation, including almost all genes involved in the production of a cell surface glycan that’s required for ...cheat sheet Read our Quick Guide to All Things Lentivirus Resources on Addgene.org Check out this guide...

....aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with... restriction site and a linker region. The PCR product is then ligated into the MCS/SacI site in pUCXKT...occur in this system if a small contaminating PCR product formed from primer dimers and containing the missing... travels along the mRNA to produce the protein product. The system, cleverly termed TRICK, for Translation...the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series of building-block...Methods, Georgieva et al describe the rational production of a new series of epitope tags, inntags (innocuous...of interest (POI). This technique requires the production and expression of fusions between the POI and...

...sites including both the edit rate and the repair product sequence. Targeted amplicon sequencing doesn’t ... for Your Experiments Get Ready-to-Use CRISPR Lentivirus ...

...their production and purification that yield high titers (see also AAV protocols for production, purification...tracing compounds either produce a colorimetric product or are fluorescent. While dyes usually spread unidirectionally...). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid which is...

...tag sequence and expect a size-shift in the PCR product for knock-in lines. Troubleshooting low knock-...our CRISPR guide pages Find ready-to-use Cas9 lentivirus ...

...common delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid...Central PMCID: PMC3161101. 20. Chang, C.J., et al., Production of embryonic and fetal-like red blood cells from...

...upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing...direction of Sabine Suppmann, the Recombinant Protein Production group at Max-Planck Institute of Biochemistry...